Cell Transplantation and Analysis Core

项目总结(见说明)： 核心C是细胞移植和分析核心，旨在为三个项目下进行的研究的细胞来源、分离和分析需求提供关键支持，核心B的具体服务目标是：目标1：从人类胚胎、胎儿、新生儿和成人组织标本中获取特定类型的细胞和组织。项目科学家将通过从胎儿、新生儿和成人样本中获取皮肤、造血细胞和间充质细胞等原始人体组织样本来满足这些组织的要求。目标2：为项目科学家提供流式细胞仪分析和分选服务。将向项目科学家提供流式细胞仪分析和荧光激活细胞分类(FACS)方面的专业知识和援助。目的3：检测免疫缺陷小鼠的畸胎瘤形成情况。诱导多能干细胞(IPS)的一个标志是它们移植到免疫缺陷小鼠体内时形成畸胎瘤的能力。将对3个项目产生的iPS细胞和分化细胞进行畸胎瘤形成能力检测，以衡量其多能性。目的：检测IPS细胞来源的候选造血干细胞(HSCs)的长期多向造血重建能力。根据项目3从IPS细胞创造的造血细胞在至少12周内重建红系、髓系和淋巴系的能力将通过移植到免疫缺陷小鼠身上进行测试。这项工作将在血液系统研究所的马库斯·穆恩奇博士的指导下进行，利用该机构已经建立的核心免疫学和细胞、组织和病毒菌核心。

PROJECT SUMMARY (See instructions): The purpose of Core C, the Cell Transplantation and Analysis Core, is to provide critical support for cell sourcing, isolation and analysis needs of research performed under the three Projects and Core B, The specific service aims of Core C are: Aim 1: To procure specific cell types and tissues from human embryonic, fetal, neonatal and adult tissue specimens. The requirements of project scientists for primary human tissue specimens such as skin, hematopoietic and mesenchymal cells will be met by obtaining these tissues from fetal, neonatal and adult specimens. Aim 2: To provide flow cytometric analysis and sorting services to project scientists. Expertise and assistance with flow cytometric analysis and fluorescence-activated cell sorting (FACS) will be provided to project scientists. Aim 3: To assay teratoma formation in immunodeficient mice. One hallmark of induced pluripotent stem (IPS) cells is their capacity to form teratomas when transplanted into immunodeficient mice. IPS cells and differentiated cells generated by the 3 projects will be assayed for teratoma forming ability to measure pluripotentiality. Aim 4: To assay long-term multilineage hematopoietic reconstitution of candidate hematopoietic stem cells (HSCs) generated from IPS cells. The capacity of hematopoietic cells created from IPS cells under Project 3 to reconstitute erythroid, myeloid and lymphoid lineages for at least 12 weeks will be tested by transplantation into immunodeficient mice. The work will be performed under the guidance of Dr. Marcus Muench at Blood systems Research Institute utilizing the Core Immunology and Cell, Tissue and Vivarium Cores already established at that institution.