EXPLORING THE ROLE OF CHOLESTEROL IN G-PROTEIN-COUPLED RECEPTOR FUNCTION, STABI

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 要求MRAC拨款总额为1，344，000 SU时间（Ranger上1，152，000 SU和漫游中192，000 SU），用于在显式脂质膜环境中运行视紫红质、5-羟色胺（5-HT 2AR）和多巴胺（D2 R）G蛋白偶联受体（GPCR）的大规模分子动力学（MD）模拟。拟议的计算将支持实现计划项目赠款的具体目标（PPG：2 P01 DA 012923 -08，H。Weinstein，PI）。该项目的长期目标是研究特定胆固醇-受体相互作用在细胞信号传导过程中GPCR功能、稳定性和寡聚化中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A MRAC grant in the total amount of 1,344,000 SU time (1,152,000 SUs on Ranger and 192,000 SUs in roaming) is requested to run large-scale Molecular Dynamics (MD) simulations of Rhodopsin, Serotonin (5-HT2AR), and Dopamine (D2R) G-protein coupled receptors (GPCRs) in explicit lipid membrane environments. The proposed calculations will support achieving the specific aims of the Program Project Grant (PPG: 2 P01 DA012923-08, H. Weinstein, PI). The long-term goal of this project is to investigate the role of specific cholesterol-receptor interactions in GPCR functioning, stability, and oligomerization during cell signaling.