STRUCTURAL DETERMINANTS OF FLICKERING IN K+ CHANNELS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们的目标是利用KCSA通道的经典轨迹来阐明选择性过滤器的短暂非导电构象状态的结构基础，这些构象状态与在单通道记录中观察到的快速闪烁有关。根据现有的实验数据，在KCSA通道的开放E71Q突变体的多S分子动力学轨迹中，闪烁预计会自然出现。这些轨迹将被用来产生关于选择性过滤器的瞬时构象的新的可实验测试的假设。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to use classical trajectories of the KcsA channel to elucidate the structural basis of the short-lived nonconductive conformational states of the selectivity filter that are associated with the rapid flickering observed in single-channel recordings. Based on available experimental data, flickering is expected to arise naturally during multi-?s molecular dynamics trajectories of the open E71Q mutant of the KcsA channel. The trajectories will be used to generate new experimentally testable hypotheses regarding the transient conformations of the selectivity filter.