Mechanisms of Botulimum Neurotoxin Action

肉毒梭菌神经毒素(BoNTs)是人类已知的毒性最强的蛋白质， 被归类为A类选择代理。他们被认为是潜在的严重威胁因素 生物恐怖主义。威斯康星大学肉毒梭菌和神经毒素核心设施是 由Eric A.Johnson领导，他的实验室在肉毒杆菌和BONT方面拥有24年的经验，是BoNTs、肉毒杆菌菌株、检测系统、动物模型和其他相关材料的高级参考来源。EAJ实验室在肉毒杆菌和BoNTs的各个方面拥有相当多的资源和专业知识。核心的主要职能是准备BoNT和相关材料，并协助GLRCE内的研究人员进行他们的研究项目。 具体目标是：菌株的获取、特性和维护(目前约为。650)肉毒梭菌和非产毒衍生菌株。 肉毒杆菌神经毒素(BoNTs)及其天然蛋白复合体的所有七种血清型和亚型的生产、纯化和鉴定。 重组BoNTs及其结构域和毒素的制备、纯化和鉴定 复合体。 动物研究的表现，包括小鼠LDSO、小鼠挑战、免疫研究和BONT中和分析。 实施非动物化验，特别是神经细胞化验，以检测和研究BoNTs和抑制剂。 维护用于肉毒杆菌和BoNTs研究的精选试剂实验室环境。 肉毒杆菌中毒核心中心将继续合作，并促进GLRCE内其他实验室的研究工作。

The Clostridium botulinum neurotoxins (BoNTs) are the most toxic proteins known to humans and are classified as Category A Select Agents. They are considered as potential serious threat agents in bioterrorism. The Clostridium botulinum and Neurotoxin Core Facility at the University of Wisconsin is directed by Eric A. Johnson, whose laboratory has 24 years of experience with C. botulinum and BoNT, and is the senior reference source for BoNTs, C. botulinum strains, assay systems, animal models, and other relevant materials. The EAJ laboratory has considerable resources and expertise in various aspects C. botulinum and BoNTs. The primary functions of the Core are preparing BoNTs and associated materials, and assisting investigators within the GLRCE in their research projects. The specific aims are: Acquisition, characterization, and maintenance of strains (currently approx. 650) of Clostridium botulinum and nontoxigenic derivative strains. Production, purification and characterization of all seven serotypes and subtypes of botulinum neurotoxins (BoNTs) and their natural protein complexes. Production, purification, and characterization of recombinant forms of BoNTs, their domains and toxin complexes. Performance of animal studies, including mouse LDSOs, mouse challenges, immunization studies, and BoNT neutralization analyses. Implementation of non-animal assays, particularly neuronal cell assays, for detection and study of BoNTs and inhibitors. Maintain Select Agent Laboratory environment for research with C. botulinum and BoNTs. The Botulism Core will continue collaborations and facilitate the research efforts of other laboratories within the GLRCE.