Study of O2 Activation by Mossbauer Spectroscopy

描述(申请人提供)：这项拟议的研究旨在阐明在核酸、氨基酸、脂肪酸的代谢、芳香化合物的生物降解和抗生素的生物合成中参与各种生化反应的含铁酶的结构和功能。这项研究将得到高价合成络合物的研究的补充，这些络合物可以作为生物系统的结构或电子模型。这些研究将集中在制备和表征高价状态的铁，如FeIV和FeV。还建议继续研究酵母线粒体，特别是产生铁离子聚集体的缺失突变体，这些聚集体与各种神经退行性疾病有关。使用的实验技术是穆斯堡尔谱和电子顺磁共振。研究计划还包括基于密度泛函理论计算的理论研究。具体地说，将研究以下系统：(1)高原生草酸2，3双加氧酶、三种Rieske双加氧酶和甲烷单加氧酶。(2)人工合成的单核FeIV和FeV络合物以及可作为铁蛋白活性中心的结构和/或电子模型的双核FeIVFeIII和FeIVFeIV络合物。(3)含有铁-四酰胺大环配体的活化剂，它们在绿色化学中具有潜力，并已被证明在杀灭炭疽孢子和消除供水中的污染物方面非常有效。(4)来自酿酒酵母的完整线粒体，以确定特定强度的穆斯堡尔谱可用于解决重要健康问题的条件。与公众健康相关的含铁酶参与氧气活化，影响所有生物系统中的许多化学转化。对这些酶的拟议研究，再加上模拟生物系统结构和功能特征的新型合成络合物的研究，将揭示这些重要的酶是如何发挥作用的。这项研究将得到一系列合成化合物的研究的补充，这些化合物已被证明在绿色化学中有效，并在杀死炭疽孢子等病原体方面非常有效。

DESCRIPTION (provided by applicant): The proposed research seeks to elucidate the structure and function of iron-containing enzymes involved in a diverse range of biochemical reactions in the metabolism of nucleic acids, amino acids, fatty acids, biodegradation of aromatic compounds, and the biosynthesis of antibiotics. This research will be complemented by the study of high-valent synthetic complexes that can serve as structural or electronic models of the biological systems. The studies will focus on preparing and characterizing high-valent states of iron such as FeIV and FeV. It is also proposed to continue studies of yeast mitochondria, in particular of deletion mutants that produce aggregates of ferric iron which have been implicated in various neurodegenerative diseases. The experimental techniques employed are M"ssbauer spectroscopy and electron paramagnetic resonance. The research plan also includes theoretical studies based on density functional theory calculations. Specifically the following systems will be studied: (1) Homoprotochatechuate 2,3 dioxygenase, three Rieske dioxygenases, and methane monooxygenase. (2) Synthetic mononuclear FeIV and FeV complexes as well as dinuclear FeIVFeIII, and FeIVFeIV complexes that can serve as structural and/or electronic models for the active sites of iron proteins. (3) Activators involving Fe-tetraamido macrocyclic ligands that have a demonstrated potential in green chemistry and have proven very potent in killing anthrax spores and eliminating pollutants from our water supply. (4) Intact mitochondria from S. cerevisiae, to identify conditions where the particular strength of M"ssbauer spectroscopy can be employed to address important health problems. PUBLIC HEALTH RELEVANCE Iron-containing enzymes involved in oxygen activation affect numerous chemical transformations in all biological systems. The proposed studies of these enzymes, coupled with investigations of novel synthetic complexes that mimic structural and functional features of the biological systems, will reveal how these important enzymes function. The research will be complemented by studies of a family of synthetic complexes that have proven to be effective in green chemistry and extremely potent in killing pathogens such as anthrax spores.