Enhanced Development of the Xiphophorus Model System

项目摘要 这份R24提案概述了资源(目标1和目标2)和研究(目标3)的组成部分，旨在 加强剑鱼遗传资源中心(XGSC)，该中心自 1930年的S；使其成为世界上最古老的活动物资源中心之一和国家科学 宝藏。XGSC目前拥有58个家系遗传系，代表24种剑鱼 1400多个水族馆中的活生生的鱼。XGSC提供了纯种鱼类和原料 来自11个国家的30多个实验室的研究人员。剑鱼的传统力量 实验模型涉及对与以下相关的遗传遗传模式的无偏见评估 复杂的表型。剑尾鱼种间高度遗传变异及生产性能 可育的种间杂交允许任何可观察到的性状遗传到个体 回交杂交后代。例如，对肿瘤易感性的基因控制已在 既有纯品系又有剑尾鱼的精选种间杂交，用于各种自发性和 诱发肿瘤，包括几种遗传上不同类型的黑色素瘤。 剑鱼基因组能力的拟议改进有望支持新的基础 了解：(A)完整生物体内细胞的整体转录状态的变化，(B) 物种间杂交带来的复杂基因相互作用，(C)遗传基础 黑色素瘤的易感性，以及(D)肿瘤形成的开始和进展。鉴于我们的初步调查 数据，我们对下一个项目期的新科学发现抱有很高的期望。具体地说， XGSC将继续为研究社区提供动物、增强的资源和用户 与遗传管理品系相关的数据库，并完全超低温保存来自 所有XGSC线路。将通过应用新的光学技术来增强基因组组件 测绘(BioNano)和超长测序(PacBio)技术。最后，我们将建立基本的 主要器官的基因表达谱，并确定通过 在外部刺激后的生物体。

Project Summary This R24 proposal outlines both resource (Aims 1 and 2) and research (Aim 3) components designed to enhance the Xiphophorus Genetic Stock Center (XGSC) that has operated within the United States since the 1930's; making it one of the oldest live animal resource centers worldwide and a national scientific treasure. The XGSC currently houses 58 pedigreed genetic lines representing 24 Xiphophorus species of live-bearing fishes in over 1,400 aquaria. The XGSC has provided pedigreed fish and materials to researchers in over 30 laboratories representing 11 countries. The traditional strength of the Xiphophorus experimental model involves the non-biased assessment of genetic inheritance patterns associated with complex phenotypes. The high genetic variability among Xiphophorus species and capability of producing fertile interspecies hybrids allows inheritance of any observable trait to be followed into individual backcross hybrid progeny. For example, genetic control of tumor susceptibility has been documented in both pure strains and in select Xiphophorus interspecies hybrids, for a variety of spontaneous and induced neoplasms, including several genetically distinct types of melanoma. Proposed improvements to Xiphophorus genomic capabilities promises to support new fundamental knowledge regarding; (a) shifts in the global transcriptional state of cells within the intact organism, (b) complex gene interactions brought about by interspecies hybridization, (c) the genetics underlying melanoma susceptibility, and (d) the initiation and progression of tumorigenesis. Given our preliminary data, we have high expectations for novel scientific findings in the next project period. Specifically, the XGSC will continuing to provide the research community with animals, enhanced resources, and user databases related to the genetically managed lines, and complete cryopreservation of germplasm from all XGSC lines. Enhancements to the genome assemblies will be made by application of new optical mapping (BioNano) and extra-long sequencing (PacBio) technologies. Finally, we will establish basal gene expression profiles for major organs and determine the rate of genetic transduction through the organism after external stimulus.