Shared Resources Core

共享资源核心：摘要 共享资源核心(SRC)被构建为支持这两项研究的物理共享资源 项目、PDX核心以及试点和跨网络项目。SRC最关键的目标是 提供PDXNet内的单个研究团队无法使用的服务和资源，或者 无法满足项目和其他部件的成本、技术和速度要求- PDTC。SRC的其他重要任务包括优化针对具体项目/样本的程序 要求，标准化化验以确保数据一致性，并采用最新的 技术。这种集中安排充分利用现有的专业知识和基础设施 我们研究所通过参与包括Htan(人类肿瘤)在内的几个大型NIH财团而发展起来 Atlas Network)、Sennet(衰老网络)、GUDMAP(生殖器发育分子解剖学 项目)、CPTAC(临床蛋白质组肿瘤分析联盟)、PE-CGS(参与者参与和 癌症基因组测序)、CRIP(联合临床影像研究计划)和PDXNet，以提高 实效和效率，避免重复劳动，减轻个别项目的压力。SRC将严格执行 与研究项目、试点项目和跨网络项目、PDX核心和生物信息学相结合 在行政核心的监督下的核心。DNA上的PDX肿瘤的详细特征， RNA和蛋白质水平将对验证、模型选择、揭示 对治疗的反应，说明治疗耐药的机制，以及确定 未来治疗试验的生物标记物和靶标。为了实现这些目标，SRC将与 研究项目和其他核心执行两种主要类型的分析：组学和成像。对于组学来说， SRC将涵盖散装和单细胞组学，包括整个外显子组测序(WES)、散装RNA-SEQ、单细胞 核RNA-seq和atac-seq(snRNA-seq和snatac-seq)、空间转录组学(Viitum)以及 全球蛋白质组学和磷酸蛋白质组学。对于成像，SRC涵盖多路目标成像 (CODEX/Phencycler)、体内临床前分子和放射成像和标准免疫荧光 (IF)染色。SRC还负责调整以适应项目不断变化的需求，以及引入 协助项目和完成中心使命的新技术和新工具。

SHARED RESOURCES CORE: SUMMARY The Shared Resources Core (SRC) is structured as a physical shared resource that will support both Research Projects, the PDX Core, as well as pilot and trans-Network projects. The most critical goal of the SRC is to provide services and resources that are either unavailable to individual research teams within PDXNet or that are unable to meet the cost, technical, and speed requirements of the Projects and other components at WU- PDTC. Other important missions for the SRC include optimization of the procedures for project/sample-specific requirements, standardization of the assays to ensure data consistency, and incorporation of the latest technologies. This centralized arrangement takes full advantage of the expertise and infrastructure existing at our institute developed through participation in several large-scale NIH consortia, including HTAN (Human Tumor Atlas Network), SenNet (Senescence Network), GUDMAP (GenitoUrinary Development Molecular Anatomy Project), CPTAC (Clinical Proteomic Tumor Analysis Consortium), PE-CGS (Participant Engagement and Cancer Genome Sequencing), CRIP (Co-Clinical Imaging Research Program), and PDXNet, in order to increase effectiveness and efficiency, avoid duplicated effort, and reduce stress on individual projects. SRC will be tightly integrated with the Research Projects, Pilot and Trans-Network Projects, the PDX Core, and the Bioinformatics Core under the supervision of the Administrative Core. Detailed characterization of the PDX tumors at the DNA, RNA, and protein levels will be important for validation, model selection, revelation of molecular changes in response to treatment, illustration of mechanisms of treatment resistance, as well as the identification of biomarkers and targets for future treatment testing. To accomplish these goals, the SRC will work with the Research Projects and other Cores to perform two main types of assays: Omics and imaging. For omics, the SRC will cover bulk and single cell omics, including whole exome sequencing (WES), bulk RNA-seq, single nucleus RNA-seq and ATAC-seq (snRNA-seq and snATAC-seq), spatial transcriptomics (Visium), as well as global proteomics and phospho-proteomics. For imaging, the SRC covers multiplex target imaging (CODEX/Phenocycler), in vivo preclinical molecular and radiologic imaging, and standard immunofluorescence (IF) staining. The SRC is also tasked with adjusting to the changing needs of the projects as well as introducing new technologies and tools to assist the Projects and to accomplish the mission of the center.