Bioinformatics Analysis of Next Generation Sequencing Data from Cancer Cells

项目摘要 该提案是对先前支持癌症生物信息学需求的R50赠款的更新。 西北大学的研究人员。最初的奖项主要支持两个NCI资助 杰出的研究人员，马库斯·彼得博士和阿里·希拉蒂法德博士，对 Robert H.卢里综合癌症中心。现在，巴汤姆博士已经成为了 癌症中心生物信息学主任，她将支持更广泛的研究 为不同的癌症中心成员提供服务。该提案的单位主任是Leon Platanias博士， 癌症中心的PI值得注意的一个具体领域将继续是马库斯·彼得博士对 6聚体种子毒性在人体对癌症的天然防御中的作用，以及这种机制如何 可以被激活以改善癌症患者的预后。她还将继续与 Simpson Querrey表观遗传学中心的同事，包括Ali Shilatifard博士， 染色质调节机制中的遗传变化如何导致表观遗传突变， 基因激活和转录延伸的缺陷。分子动力学研究 感染卡波西肉瘤相关疱疹病毒的原代人类细胞培养物将继续进行， 博士伊娃戈特温，让我们深入了解卡波西肉瘤如何在艾滋病患者中出现。所有这三 研究人员是癌症中心的成员，他们的研究得到了癌症中心P30基金的支持。 所有这些项目的共同点是需要仔细的，生物动机的计算 分析，使下一代测序数据的意义，这是必不可少的检查功能， 细胞，在癌症发展之前和之后以及药物扰动之前和之后。博士 Bartom将提供这种分析，她在生物学和计算方面的专业知识将允许癌症 生物学家从他们的数据中提取更多的洞察力，并保持高标准的科学严谨性， 再现性

Project Summary This proposal is a renewal of a prior R50 grant that supported the bioinformatics needs of cancer researchers at Northwestern University. The initial award primarily supported two NCI funded Outstanding Investigators, Dr. Marcus Peter and Dr. Ali Shilatifard, with a small fraction of support for the Robert H. Lurie Comprehensive Cancer Center. Now, Dr. Bartom has become the Scientific Associate Director for Bioinformatics for the Cancer Center, and she will support a broader array of research programs for different cancer center members. The Unit Director for this proposal is Dr. Leon Platanias, PI of the Cancer Center. A specific area of note will continue to be Dr. Marcus Peter’s investigations into the role of 6mer seed toxicity in the body’s natural defenses against cancer, and how this mechanism can be activated in order to improve outcomes for cancer patients. She will also continue to work with colleagues within the Simpson Querrey Center for Epigenetics, including Dr. Ali Shilatifard, to understand how genetic changes in the machinery of chromatin regulation can lead to epigenetic mutations and defects in gene activation and transcriptional elongation. Research into the molecular dynamics in primary human cell cultures infected with Kaposi’s Sarcoma-associated Herpesvirus will continue with Dr. Eva Gottwein, giving insight into how Kaposi’s Sarcomas arise in AIDS patients. All three of these investigators are cancer center members, whose research is supported by the Cancer Center P30 grant. The common thread in all of these projects is the need for careful, biologically motivated computational analysis to make sense of next generation sequencing data that is essential in examining the function of cells, both before and after cancer development and before and after pharmaceutical perturbation. Dr. Bartom will provide this analysis, and her expertise in both biology and computation will allow cancer biologists to extract more insight from their data, and to maintain a high standard of scientific rigor and reproducibility.