Virus Production & Molecular Biology Core

病毒生产与分子生物学核心 项目摘要 病毒生产和分子生物学核心模块将提供将现代 分子和基因技术被纳入耶鲁大学视觉研究研究人员的项目。第一，核心 模块将为从事转基因工作的实验室提供病毒准备协助和相关服务 动物和/或病毒技术。第二，核心模块将对新的调查人员和学生进行基本培训 分子生物学技术，包括DNA修饰以产生新的病毒载体，以及 组织学技术，如免疫染色。第三，它提供和维护一系列分子 生物设备将在自己没有此类设备的视觉研究人员之间共享 实验室。病毒载体是在终末分化细胞中传递基因的强大工具。例如, 腺相关病毒(AAVs)在视觉研究中的应用日益广泛 它们的许多有利特征包括缺乏致病性、低免疫原性和能够 在神经元中维持长期的转基因表达。病毒生产&分子生物学的核心 该模块将能够提供AAV载体的设计和生产，以满足各种研究 耶鲁大学视觉研究人员的需求。病毒设计和生产的内部能力将 通过生产定制的、高滴度的AAV产品，显著增强视觉研究 商业消息来源。将扩大分子、遗传和组织学技术方面的咨询和培训 在这些领域缺乏足够背景的视觉研究人员的研究能力 针对特定研究需求的面对面咨询的便利性。

Virus Production & Molecular Biology Core Project Summary The Virus Production & Molecular Biology Core module will provide the means to incorporate modern molecular and genetic techniques into the programs of vision research investigators at Yale. First, the Core module will supply virus preparation assistance and related services for laboratories working with transgenic animals and/or viral techniques. Second, the Core module will train new investigators and students in basic molecular biology techniques, including DNA modification to generate new viral vectors, as well as histological techniques, such as immunostaining. Third, it provides and maintains a range of molecular biology equipment to be shared among vision researchers who lack such equipment in their own laboratories. Viral vectors are powerful tools for gene delivery in terminally differentiated cells. For example, adeno-associated viruses (AAVs) have enjoyed increasingly wide application in vision research based on their many favorable features that include the lack of pathogenicity, low immunogenicity, and the ability to maintain long-term transgene expression in neurons. The Virus Production & Molecular Biology Core module will be capable of providing both AAV vector design and production to meet a variety of research needs among vision researchers at Yale. An in-house capability for virus design and production will significantly enhance vision research by producing custom, high-titer AAV products not available from commercial sources. Consultation and training in molecular, genetic and histological techniques will expand the research capabilities of vision researchers that lack sufficient background in these areas with the convenience of in-person consultation for specific research needs.