TOPIC 382 - INTEGRATED IMAGING AND MOLECULAR ANALYSIS OF GLIOBLASTOMA USING SCOPE SEQ ON THE AIR FLOW

在细胞的基础上结合基因组分析和高分辨率成像通常依赖于定制的技术，这往往需要大量的专业知识和资金成本。细胞微系统公司与哥伦比亚大学博士彼得·西姆斯合作，将一种用于单细胞转录分析的集成成像和样品制备的新工作流程商业化。SIMS博士的工作流程，单细胞光学表型和表达测序(SCOPEESeq)，将在细胞微系统公司正在开发的一种新的微流控仪器AIREFLOW上实现自动化。该系统除了提供单细胞转录分析的全自动样品制备(即scERNAESeq)外，还提供单细胞的详细图像分析。在这里，我们将应用这个合作开发的平台来研究难治性癌症--胶质母细胞瘤。第一阶段计划将使用胶质母细胞瘤神经球作为模型系统，以验证该系统在以下方面的能力：功能染料成像、单细胞分子分析以及在细胞EbyEcell的基础上连接这些数据模式。在拟议的第二阶段，这些能力将在患者的活检组织中进一步测试，以测试在脑肿瘤手术切除中使用的荧光染料的肿瘤细胞特异性。我们预计，由此产生的系统将在这一领域产生重大影响，并在更广泛的市场上具有相当大的商业潜力。

Combining both genomic analysis and highEresolution imaging on a cellEbyEcell basis typically relies on customized technology which often requires significant expertise and capital cost. Cell Microsystems, Inc. has partnered with Peter Sims, PhD of Columbia University to commercialize a novel workflow for integrated imaging and sample preparation for single cell transcriptomic analysis. Dr. Sims workflow, Single Cell Optical Phenotyping and Expression Sequencing (SCOPEESeq), will be automated on a new microfluidic instrument under development by Cell Microsystems, the AIREFLOW. The system provides detailed image analysis of single cells, in addition to fullyEautomated sample preparation of single cell transcriptomic analysis (i.e. scERNAESeq). Here, we will apply this collaboratively developed platform to investigating the intractable cancer, glioblastoma. The Phase I program will use glioblastoma neurospheres as a model system to validate the system’s capabilities for: functional dye imaging, single cell molecular analysis and linking these data modalities on a cellEbyEcell basis. During the proposed Phase II these capabilities will be further tested in patientEderived biopsies to test the tumor cell specificity of fluorescent dyes used during surgical brain tumor resection. We expect the resulting system to have significant impact in this field, as well as considerable commercial potential in the broader market.