Cross-sectional associations of oral microbiota with oral HPV infection among high-risk Hispanic adults

项目摘要 在美国，人乳头瘤病毒（HPV）阳性口咽癌的发病率上升是一个重要的因素。 公共卫生问题，而西班牙裔显示这种恶性肿瘤的负担增加。最近的研究表明 口咽癌病例和对照之间以及HPV+和HPV-之间的口腔微生物群差异 个体尽管如此，评估口腔微生物群对口腔HPV感染的作用的研究， 对于口咽癌来说，这是稀缺的，对于制定有效的预防策略至关重要。拟议 一项横断面研究将从波多黎各的性传播感染（STI）诊所招募300名参与者。 本申请的具体目的是（1）表征具有和不具有口腔微生物群的患者的口腔微生物群概况。 口腔HPV感染，和（2）评估物种水平的口腔微生物群和口腔HPV感染之间的关联。 参与者将提供唾液样本用于口腔HPV检测，遵循NHANES方法，并用于口腔HPV检测。 微生物分析将通过以下方式收集社会人口统计学、行为、牙科和医学特征 一份由面试官填写的问卷。口腔HPV感染的危险因素将使用音频收集 计算机辅助自我访谈（ACASI）方法，收集敏感的风险行为数据的有用工具。 HPV分型将使用38种类型特异性探针通过斑点杂交进行，包括致癌和 非致癌类型，而对于微生物群，将进行基因组DNA提取和16 S rDNA V4扩增， 完了然后对样品进行Illumina Miseq测序和数据分析。据我们所知，这 是第一项评估口腔微生物群与口腔HPV感染之间关系的研究， 同质的西班牙裔人口。越来越多的证据支持这种联系的生物学可解释性 在各种解剖部位和口腔癌中的微生物群和HPV感染之间存在联系;然而， 与口腔HPV感染的关系有限。口腔菌群在口腔HPV感染中的作用 可以为制定预防性公共卫生战略提供必要的信息， 表明口腔微生物群调节口腔HPV感染和口腔癌发生，这对口腔癌的影响， 癌症预防和控制战略可能很重要。

PROJECT SUMMARY The rising rates of Human Papilloma Virus (HPV)-positive oropharyngeal cancers in the U.S. is a significant public health issue, while Hispanics show increased burden of this malignancy. Recent research suggests differences in oral microbiota between oropharyngeal cancer cases and controls, and between HPV+ and HPV- individuals. Nonetheless, research evaluating the role of oral microbiota on oral HPV infection, a main risk factor for oropharyngeal cancer, is scarce, and essential to develop effective prevention strategies. The proposed cross-sectional study will recruit 300 participants from sexually transmitted infections (STI) clinics of Puerto Rico. The specific aims of this application are to (1) characterize the oral microbiota profiles of patients with and without oral HPV infection, and (2) assess the association between species-level oral microbiota and oral HPV infection. Participants will provide saliva samples for oral HPV detection, following the NHANES methodology, and for oral microbiota analysis. Socio-demographic, behavioral, dental, and medical characteristics will be collected through an interviewer-administered questionnaire. Risk factors for oral HPV infection will be collected using the audio computer-assisted self-interview (ACASI) methodology, a useful tool for collecting sensitive risk behavior data. HPV typing will be performed by dot-blot hybridization using 38 type-specific probes, including oncogenic and non-oncogenic types, while for microbiota, genomic DNA extractions and 16S rDNA V4 amplifications will be done. Samples will then be subjected to Illumina Miseq sequencing and data analyses. To our knowledge, this is the first study that will assess the association between the oral microbiota and oral HPV infection among a homogenous Hispanic population. Accumulating evidence supports a biological plausibility for the association between the microbiota and HPV infection in various anatomical sites and with oral cancer; however, data on its relationship with oral HPV infection is limited. The identification of the oral microbiota’s role on oral HPV infection may provide essential information for the development of preventive public health strategies, as if research shows that the oral microbiota modulates oral HPV infection and oral carcinogenesis, the implications for oral cancer prevention and control strategies might be important.