A novel in vivo functional imaging platform to study the re-animation of light responses in a blind eye

摘要 合成偶氮苯光开关可以绕过失明视网膜的退化杆和锥光感受器 并直接光敏视网膜神经节细胞（RGC）以恢复光反应。这份提案概述了一部小说 利用双光子跟踪扫描激光检眼镜（2 P-tSLO）和GCaMP 6的体内成像平台 Ca 2+成像以可视化具有恢复的光敏感性的盲rd 1小鼠的RGC。该研究计划在 该奖学金需要1）使用Ca 2+成像开发优化的光开关传递系统， 多电极阵列电生理学和2）使用体内Ca 2+评价光开关光恢复 显像

Abstract Synthetic azobenzene photoswitches can bypass the degenerated rod and cone photoreceptors of a blind retina and directly photosensitize retinal ganglion cells (RGCs) to restore light-response. This proposal outlines a novel in vivo imaging platform that utilizes 2-photon tracking scanning laser ophthalmoscopy (2P-tSLO) and GCaMP6 Ca2+ imaging to visualize RGCs of blind rd1 mice that have reanimated light sensitivity. The research planned in this fellowship entails 1) the development of an optimized photoswitch delivery system using Ca2+ imaging and multielectrode array electrophysiology and 2) the evaluation of photoswitch light-restoration using in vivo Ca2+ imaging.