Tissue Culture and Hybridoma Core

组织培养和杂交单元的目的是提高个人的能力， 研究人员，其与视觉相关的研究涉及使用细胞培养物和单克隆抗体。的 模块由经验丰富的人员操作和监督，并为 以下具体目的：1）协助研究者从眼组织中分离原代细胞; 2）维持 和传代建立的细胞系; 3）进行细胞系的大规模扩增; 4）进行瞬时 细胞转染; 5）产生稳定转染的细胞系; 6）为以下人员提供战略咨询和培训： 计划细胞培养实验和/或杂交瘤生产的实验室人员; 7）生产， 分离和储存杂交瘤; 8）纯化单克隆抗体; 9）进行支原体检测;和10） 进行细胞的低温保存和储存。该模块帮助VSRC调查人员生成独特的 试剂，以控制其细胞培养实验和单克隆抗体生产的所有阶段，以及 最终以具有成本效益的方式进行尖端的视觉相关研究。

The purpose of the Tissue Culture and Hybridoma Module is to enhance the capabilities of individual investigators, whose vision-related research involves the use of cell cultures and monoclonal antibodies. The Module is operated and supervised by highly experienced personnel and provides infrastructure for the following Specific Aims: 1) to assist investigators in isolation of primary cells from ocular tissues; 2) to maintain and passage established cell lines; 3) to perform large scale amplification of cell lines; 4) to conduct transient cell transfection; 5) to generate stably transfected cell lines; 6) to provide strategic advice to and training of laboratory personnel in planning of cell culture experiments and/or hybridoma production; 7) to produce, isolate, and store hybridomas; 8) to purify monoclonal antibodies; 9) to perform mycoplasma testing; and 10) to perform cryogenic preservation and storage of cells. The Module helps VSRC investigators to generate unique reagents, to control all phases of their cell culture experiments and monoclonal antibody production, and ultimately to conduct cutting-edge vision-related research in a cost-efficient manner.