Tissue Culture and Hybridoma Core

组织培养和杂交瘤模块的目的是提高个体的能力 研究人员，他们的视力相关研究涉及使用细胞培养和克隆抗体。这个 模块由经验丰富的人员操作和监督，并为 具体目标如下：1)协助研究人员从眼组织中分离出原代细胞；2)保持 并传代建立细胞系；3)对细胞系进行大规模扩增；4)进行瞬时 细胞转染法；5)建立稳定的转染性细胞系；6)提供战略建议和培训 计划细胞培养实验和/或杂交瘤生产的实验室人员；7)生产， 分离、储存杂交瘤；8)纯化单抗；9)进行支原体检测；10) 对细胞进行低温保存和储存。该模块帮助VSRC调查人员生成独特的 试剂，以控制其细胞培养实验和单抗产生的所有阶段，以及 最终以具有成本效益的方式进行与视觉相关的尖端研究。

The purpose of the Tissue Culture and Hybridoma Module is to enhance the capabilities of individual investigators, whose vision-related research involves the use of cell cultures and monoclonal antibodies. The Module is operated and supervised by highly experienced personnel and provides infrastructure for the following Specific Aims: 1) to assist investigators in isolation of primary cells from ocular tissues; 2) to maintain and passage established cell lines; 3) to perform large scale amplification of cell lines; 4) to conduct transient cell transfection; 5) to generate stably transfected cell lines; 6) to provide strategic advice to and training of laboratory personnel in planning of cell culture experiments and/or hybridoma production; 7) to produce, isolate, and store hybridomas; 8) to purify monoclonal antibodies; 9) to perform mycoplasma testing; and 10) to perform cryogenic preservation and storage of cells. The Module helps VSRC investigators to generate unique reagents, to control all phases of their cell culture experiments and monoclonal antibody production, and ultimately to conduct cutting-edge vision-related research in a cost-efficient manner.