Tissue Culture and Hybridoma Core
Tissue Culture and Hybridoma Core
批准号:
10001527
负责人:
Robert Gerd Salomon
金额:
$19.36万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-04-01 至 2022-08-31
关键词:
Cell Culture TechniquesCell LineCellsCore GrantHuman ResourcesHybridomasIndividualInfrastructureLaboratory PersonnelMonoclonal AntibodiesMycoplasmaPhaseReagentResearchResearch PersonnelSupervisionTestingTissuesTransfectionVisionVision researchcost efficientcryogenicsestablished cell lineexperienceexperimental studyhybridoma productionlaboratory experiencemonoclonal antibody productionpreservationranpirnasetissue culture
中文摘要
组织培养和杂交单元的目的是提高个人的能力,
研究人员,其与视觉相关的研究涉及使用细胞培养物和单克隆抗体。的
模块由经验丰富的人员操作和监督,并为
以下具体目的:1)协助研究者从眼组织中分离原代细胞; 2)维持
和传代建立的细胞系; 3)进行细胞系的大规模扩增; 4)进行瞬时
细胞转染; 5)产生稳定转染的细胞系; 6)为以下人员提供战略咨询和培训:
计划细胞培养实验和/或杂交瘤生产的实验室人员; 7)生产,
分离和储存杂交瘤; 8)纯化单克隆抗体; 9)进行支原体检测;和10)
进行细胞的低温保存和储存。该模块帮助VSRC调查人员生成独特的
试剂,以控制其细胞培养实验和单克隆抗体生产的所有阶段,以及
最终以具有成本效益的方式进行尖端的视觉相关研究。
英文摘要
The purpose of the Tissue Culture and Hybridoma Module is to enhance the capabilities of individual
investigators, whose vision-related research involves the use of cell cultures and monoclonal antibodies. The
Module is operated and supervised by highly experienced personnel and provides infrastructure for the
following Specific Aims: 1) to assist investigators in isolation of primary cells from ocular tissues; 2) to maintain
and passage established cell lines; 3) to perform large scale amplification of cell lines; 4) to conduct transient
cell transfection; 5) to generate stably transfected cell lines; 6) to provide strategic advice to and training of
laboratory personnel in planning of cell culture experiments and/or hybridoma production; 7) to produce,
isolate, and store hybridomas; 8) to purify monoclonal antibodies; 9) to perform mycoplasma testing; and 10) to
perform cryogenic preservation and storage of cells. The Module helps VSRC investigators to generate unique
reagents, to control all phases of their cell culture experiments and monoclonal antibody production, and
ultimately to conduct cutting-edge vision-related research in a cost-efficient manner.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Glutathionylated Products of Radical-Induced Lipid Oxidation in Inflammatory Disease
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批准号:10736332
-
项目类别:
-
资助金额:$39.45万
-
财政年份:2023
-
负责人:Robert Gerd Salomon
-
依托单位:
Preprostaglandin Endoperoxides
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批准号:8102238
-
项目类别:
-
资助金额:$25.76万
-
财政年份:2010
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:8055311
-
项目类别:
-
资助金额:$29.84万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
REACTIVE INTERMEDIATES OF OXIDATIVE LIPID FRAGMENTATION
-
批准号:9114118
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项目类别:
-
资助金额:$38.36万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:7415052
-
项目类别:
-
资助金额:$25.18万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:7227456
-
项目类别:
-
资助金额:$25.55万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
REACTIVE INTERMEDIATES OF OXIDATIVE LIPID FRAGMENTATION
-
批准号:9321185
-
项目类别:
-
资助金额:$38.31万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:8464119
-
项目类别:
-
资助金额:$28.35万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:7649632
-
项目类别:
-
资助金额:$33.54万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:8266464
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项目类别:
-
资助金额:$29.84万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:7102418
-
项目类别:
-
资助金额:$28.68万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Reactive Intermediates of Oxidative Lipid Fragmentation
-
批准号:7805441
-
项目类别:
-
资助金额:$31.09万
-
财政年份:2006
-
负责人:Robert Gerd Salomon
-
依托单位:
Tissue Culture and Hybridoma Core
-
批准号:10244970
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项目类别:
-
资助金额:$19.36万
-
财政年份:1997
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负责人:Robert Gerd Salomon
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依托单位:
Molecular Basis of Oxidative Modification of LDL
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批准号:7394968
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项目类别:
-
资助金额:$40.6万
-
财政年份:1996
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负责人:Robert Gerd Salomon
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依托单位:
Molecular Basis of Oxidative Modification of LDL
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批准号:7589773
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项目类别:
-
资助金额:$42.64万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
MOLECULAR BASIS OF OXIDATIVE MODIFICATION OF LDL
-
批准号:2415643
-
项目类别:
-
资助金额:$25.02万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
MOLECULAR BASIS OF OXIDATIVE MODIFICATION OF LDL
-
批准号:2231169
-
项目类别:
-
资助金额:$25.34万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
Molecular Basis of Oxidative Modification of LDL
-
批准号:7858072
-
项目类别:
-
资助金额:$43.01万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
MOLECULAR BASIS OF OXIDATIVE MODIFICATION OF LDL
-
批准号:2910571
-
项目类别:
-
资助金额:$27.06万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
MOLECULAR BASIS OF OXIDATIVE MODIFICATION OF LDL
-
批准号:2702265
-
项目类别:
-
资助金额:$26.08万
-
财政年份:1996
-
负责人:Robert Gerd Salomon
-
依托单位:
海外基金