Animal Facility

文摘：动物设施(AF) UPMC Hillman癌症中心(HCC)的基础和转化性癌症研究被广泛关注 肿瘤进展的分子理解与新的预防和治疗方法的发展 (药理制剂、细胞和基因疗法)使用各种癌症的体外和体内模型。 了解从单个分子到整个动物的生物过程是至关重要的，而动物设施 (AF)是一个基本的共享资源，负责教育调查人员并提供资源、专业知识、 以及当这些科学调查需要动物模型时的服务。当新的化疗药物 试剂、诊断方式或预防措施在体外或理论上显示出希望 方法论，翻译的一个关键基础步骤是使用动物模型。这通常是在临床之前需要的 评估。为了开发和测试癌症预防或治疗方法，动物研究可以 需要描述肿瘤的反应性、生存曲线、组织病理学变化和发现 以及机械生物标记物的验证。动物研究使研究团队能够研究一种 通过测量肿瘤负担或免疫效应细胞向肿瘤募集的减少来进行干预 体内微环境。AF为所有研究项目的研究人员提供了健康的 动物(小鼠、大鼠和麝鼠)，转基因品种，适当的畜牧业，兽医支持， 兽医病理学家的专业知识和屏障条件下的新型专用设备(如伽马) 照射器、成像设备)，允许对体内事件进行多参数和定量分析 可重现的方式和效率。

Abstract: Animal Facility (AF) Basic and translational cancer investigations at the UPMC Hillman Cancer Center (HCC) are broadly focused on molecular understanding of cancer progression and development of novel preventatives and therapeutics (pharmacological agents, cell-based, and gene therapies) using in vitro and in vivo models of various cancers. Understanding biological processes from single molecule to whole animal is critical, and the Animal Facility (AF) is an essential shared resource charged with educating investigators and providing resources, expertise, and services when these scientific investigations require animal modeling. When new chemotherapeutic agents, diagnostic modalities, or preventive measures show promise using in vitro or theoretical methodologies, a key basic to translational step is use of animal models. This is often required prior to clinical evaluations. For development and testing of cancer prevention or treatment approaches, animal studies may be required to characterize the tumor responsiveness, survival curves, histopathologic changes, and discovery and validation of mechanistic biomarkers. Animal studies allow research teams to study the effectiveness of an intervention by measuring decreases in tumor burden or recruitment of immune effector cells to the tumor microenvironment in vivo. The AF provides investigators across all research programs access to healthy animals (mice, rats and musk shrews), transgenic strains, appropriate husbandry, veterinary support, veterinary pathologist expertise and novel dedicated equipment under barrier conditions (e.g., gamma irradiators, imaging devices) that allow multi-parametric and quantitative analyses of events in vivo in a highly reproducible manner and with efficiency.