Animal Facility

翻译后摘要：动物设施（AF） UPMC希尔曼癌症中心（HCC）的基础和转化癌症研究广泛关注 对癌症进展的分子理解和新型预防和治疗药物的开发 （药理学试剂、基于细胞的和基因疗法）使用各种癌症的体外和体内模型。 了解从单个分子到整个动物的生物过程至关重要， (AF)是一个重要的共享资源，负责教育调查人员，提供资源，专业知识， 当这些科学研究需要动物模型时，当新的化疗药物 药物、诊断方式或预防措施显示出使用体外或理论 方法学中，转化步骤的关键基础是使用动物模型。这通常需要在临床之前进行。 评价。对于癌症预防或治疗方法的开发和测试，动物研究可以 需要描述肿瘤反应性、生存曲线、组织病理学变化和发现 和机制生物标志物的验证。动物研究使研究小组能够研究 通过测量肿瘤负荷的降低或免疫效应细胞向肿瘤的募集来进行干预 体内微环境AF为所有研究项目的研究者提供健康的 动物（小鼠、大鼠和麝香鼠）、转基因品系、适当的饲养、兽医支持， 兽医病理学家的专业知识和在屏障条件下的新型专用设备（例如，伽马 辐射器、成像设备），其允许在高度生物学特性的情况下对体内事件进行多参数和定量分析。 可重复的方式和效率。

Abstract: Animal Facility (AF) Basic and translational cancer investigations at the UPMC Hillman Cancer Center (HCC) are broadly focused on molecular understanding of cancer progression and development of novel preventatives and therapeutics (pharmacological agents, cell-based, and gene therapies) using in vitro and in vivo models of various cancers. Understanding biological processes from single molecule to whole animal is critical, and the Animal Facility (AF) is an essential shared resource charged with educating investigators and providing resources, expertise, and services when these scientific investigations require animal modeling. When new chemotherapeutic agents, diagnostic modalities, or preventive measures show promise using in vitro or theoretical methodologies, a key basic to translational step is use of animal models. This is often required prior to clinical evaluations. For development and testing of cancer prevention or treatment approaches, animal studies may be required to characterize the tumor responsiveness, survival curves, histopathologic changes, and discovery and validation of mechanistic biomarkers. Animal studies allow research teams to study the effectiveness of an intervention by measuring decreases in tumor burden or recruitment of immune effector cells to the tumor microenvironment in vivo. The AF provides investigators across all research programs access to healthy animals (mice, rats and musk shrews), transgenic strains, appropriate husbandry, veterinary support, veterinary pathologist expertise and novel dedicated equipment under barrier conditions (e.g., gamma irradiators, imaging devices) that allow multi-parametric and quantitative analyses of events in vivo in a highly reproducible manner and with efficiency.