Biomimetic Matrix-Based Multiphasic System for Rotator Cuff Repair

项目摘要 肩袖肌腱损伤是一种非常常见的肌肉骨骼损伤，有300多万人受伤 每年在美国和欧盟接受治疗。这些损伤的愈合和修复有限。 可能由于血管形成不良，阻碍营养物质和肌腱细胞的充分输送而产生 新的组织。近年来，许多组织工程学方法被用于修复肩袖损伤。 然而，由于目前缺乏可用的生物材料，成功的产品并不多。为了进一步 在这一领域的发展，并克服现有设备的限制，我们提出了一个仿生矩阵 基于多阶段支架设计，可与现有缝合锚结合使用，以加强修复 和肩袖吻合部的再生。在这项提案中，我们将进一步进行体外评估 支架的细胞相容性，并用小动物评价支架的体内性能 肩袖修复模型。目标1将确定最佳多相脚手架参数，以促进区域- 在肩袖中形成特殊的末端组织。目标2将评估区域的生物学性能- 使用共培养模型和动物肩袖修复模型的特定多相支架。此数据将 为我们今后设计用于翻译研究的大型动物模型提供有价值的参考 商业化。最后，本研究将一种新型的多相生物材料支架推向临床 肩袖撕裂的治疗实施有可能迅速转化为临床试验和 练习一下。

Project Summary Rotator cuff tendon injury is a very most common musculoskeletal injury, with over 3 million injuries being treated in the United States and European Union each year. These injuries have limited healing and repair potential due to poor vascularization, preventing adequate delivery of nutrients and tendon cells to produce new tissue. Recently, many tissue engineering approaches have been introduced to repair rotator cuff injuries. However, not many products have succeeded due to the lack of the currently available biomaterials. To further development in this area and overcome limitations with current devices, we propose a biomimetic matrix based-multiphasic scaffold designed to be used in conjunction with existing suture anchors to enhance repair and regeneration of the rotator cuff enthesis. In this proposal, further, we will perform in vitro evaluations of cytocompatibility of the scaffolds, and evaluate the in vivo performance of the scaffolds using a small animal rotator cuff repair model. Aim 1 will determine optimal multiphasic scaffold parameters to promote zone- specific enthesis tissue formation in rotator cuff. Aim 2 will evaluate the biological performances of zone- specific multiphasic scaffolds using a co-culture model and an animal rotator cuff repair model. This data will provide valuable references for our future design of a large animal model for translational research and commercialization. Finally, this study will advance a novel multiphasic biomaterial scaffold towards clinical implementation for treatment of rotator cuff tears with the potential for rapid translation to clinical trials and practice.