Animal and Bioinformatics Core

项目摘要 动物核心(核心C)将通过产生所有的老鼠来提供集中服务 中心项目所需的。核心中心集中供应鼠标将确保 具有相同遗传背景的小鼠被用来最小化潜在的 表型、基因组或表观基因组分析。核心员工状况良好的事实 接受过HFD治疗方面的培训，熟练地监测发展和健康状况； GTT和ITT的测量将确保一致的数据质量和可重复性。 具体来说，核心C将产生高脂肪饮食(HFD)和正常饮食(ND)喂养的野生型 和转基因小鼠，收集表型数据并准备精子和组织样本 两项基本工程。项目2和项目3都将针对HFD和/或不活动的影响开展工作 精子表观基因组与后代健康。WT或转基因(TU示踪剂和 KitcopGFP)小鼠将接受HFD或ND为期3个月的治疗，项目3也需要 通过给HFD处理的小鼠喂食ND并引入 转向盘。精子表观基因组改变对HFD反应的影响 和/或运动，处理的雄性小鼠需要与WT雌性小鼠饲养才能产生 F1子代。核心C将维持小鼠群体，执行所有治疗，收集 表型数据并提供实验所需的精子和组织样本 在项目2和3中提议。

Project Summary The Animal Core (Core C) will provide centralized services by generating all the mice needed for the Center projects. A centralized supply of mice by the Core will ensure that mice with the same genetic background are used to minimize potential variations in phenotypic, genomic or epigenomic analyses. The fact that Core personnel are well trained for HFD treatment, skillful in monitoring development and health status, and measurement of GTT and ITT will ensure consistent data quality and reproducibility. Specifically, Core C will generate high fat diet (HFD)- and normal diet (ND)-fed wild-type and transgenic mice, collect phenotypic data and prepare sperm and tissue samples for the two basic projects. Both projects 2 and 3 will work on effects of HFD and/or inactivity on sperm epigenome and offspring health. Both WT or transgenic (TU tracer and KitcopGFP) mice will be on a HFD or a ND for 3 months, and Project 3 also needs to reverse the HFD effect by feeding HFD-treated mice with ND and introducing to a running wheel. To analyze the effects of altered sperm epigenome in response to HFD and/or exercise, the treated male mice need to be bred with WT female mice to generate F1 progeny. Core C will maintain the mouse colonies, perform all the treatments, collect phenotypic data and provide sperm and tissue samples needed for the experiments proposed in Projects 2 and 3.