Development of an innovative approach for in situ treatment of PCB impacted sediments by microbial bioremediation

项目摘要 多氯联苯（PCBs）是遗留污染物中最具问题的污染物之一。 多氯联苯在环境中具有持久性和移动的性，在沉积物中普遍存在， 美国工业区的水生系统。其毒性相对较高， 生物累积的可能性会增加对人体和生态受体的风险。因此，在本发明中， 多氯联苯往往是受影响沉积物地点的主要风险驱动因素。常见做法 修复受多氯联苯影响的沉积物的成本很高，通常需要物理去除表面活性剂 沉积物，用多层工程帽覆盖沉积物或疏浚洼地，以及 把受污染的沉积物弃置于密封堆填区。 一个新兴的策略，有效地去除多氯联苯从沉积物原位是使用生物改良 活性炭（AC），它接种AC小球与富集培养物的多氯联苯降解 微生物这项研究的共同研究者进行了基础研究 支持使用生物改良活性炭来修复沉积物中的多氯联苯，并已开发和 获得专利的商业上可行的方法，用于培养，培养和输送接种的AC 颗粒到沉淀物。然而，在使用生物改良剂进行中试规模研究期间， AC，两个因素，将限制准备使用这项技术的大型，多英亩的网站是 确定：1）厌氧PCB降解菌的大规模生长、储存和运输， 和; 2）在应用期间用于CABAC颗粒的大规模方法。拟议研究 旨在解决这些局限性。 拟议的研究将测试使用时间释放生长培养这些生物体的方法 媒体，并开发和测试存储容器，保持最佳的环境条件， 厌氧微生物的长期生存能力。这些进展最终将允许交付 为大型项目提供大量的多氯联苯降解微生物。拟议研究 还将开发和测试方法，用于连续，均匀接种高容量的AC 颗粒与多氯联苯降解微生物，这将允许成本效益的应用， 多英亩的受多氯联苯影响的场地。结合在一起，拟议的研究预计将产生一个 将这一技术从试验规模直接转移到完全商业可行性。 1

Project Summary Polychlorinated biphenyls (PCBs) are one of the most problematic of legacy contaminants. Persistent and mobile in the environment, PCBs are largely ubiquitous in depositional sediments of aquatic systems in industrial regions of the United States. Their relatively high toxicity and bioaccumulation potential cause elevated risk to both human and ecological receptors. As such, PCBs are often the primary risk driver at impacted sediment sites. Common practices for remediating PCB-impacted sediments are costly, often involving the physical removal of surficial sediments, capping the sediments or dredge depression with a multi-layered engineered cap, and disposal of the contaminated sediments in a confined landfill. An emerging strategy for effectively removing PCBs from sediments in-situ is the use of bioamended activated carbon (AC), which inoculates AC pellets with enriched cultures of PCB-degrading microbes. The co-investigators of this proposed research have performed the fundamental research behind the use of bioamended AC for remediation of PCBs from sediment and have developed and patented commercially-viable methods for growing, inoculating, and delivering the inoculated AC pellets to sediments. However, during the performance of pilot-scale studies using the bioamended AC, two factors that would limit the ready use of this technology for large, multi-acre sites were identified: 1) the large-scale growth, storage, and transport of anaerobic PCB degrading bacteria, and; 2) large-scale methods for inoculating AC pellets during application. The proposed research aims to address these limitations. The proposed research will test methods of culturing these organisms using time-release growth media, and develop and test storage vessels that maintain optimal environmental conditions for the long-term viability of the anaerobic organisms. These advances will ultimately allow for the delivery of large volumes of PCB degrading microorganisms for large-scale projects. The proposed research will also develop and test methods for the continuous, uniform inoculation of high volumes of AC pellets with the PCB-degrading microorganisms, which will allow for cost-effective application at multi-acre PCB-impacted sites. Coupled together, the proposed research is anticipated to result in a direct transfer of this technology from pilot-scale to full commercial viability. 1