High-throughput Biocatalyst Manufacturing for Environmental Biotechnology

项目摘要/摘要 各种危险化合物继续对公众健康和安全构成严重和广泛的威胁。 美国。有机化合物，特别是氯化溶剂、碳氢化合物、消毒 副产品、杀虫剂、多环芳烃(PAHs)、多氯联苯(PCbs)和其他 异生(非自然产生)有机化学品是水污染物的一大类。这些 高优先级污染物通常由州和联邦机构监管，因为它们已被证明与癌症和 各种疾病，包括肝脏或肾脏疾病，免疫功能障碍，神经系统疾病，以及 荷尔蒙或生殖缺陷。然而，现有的化学、物理和生物技术 这些化合物受到严重的限制，导致处理效率低和/或增加成本 适用于超级基金网站经理、市政当局和供水部门。 该项目基于之前的第二阶段项目，涉及到成功开发增强型 基于共代谢的生物技术，显著克服了传统治疗的局限性 系统。这项新技术已被证明可以将有害有机化合物降解为 无害的副产品，而不是产生集中的二次废物流。此外，这一新的 与化学或紫外线相比，技术显著降低了能源和维护成本 氧化，并在一系列操作条件下提供就地和异地处理选项 同时以经济高效的方式从水中去除有害有机化合物。 在这项提议中，这项技术的基础制造工艺在以下方面得到了显著改进 制造产能、减少废物、质量控制和环境足迹。具体而言，现有的 该技术的生产流程被重新设计和重新设计，使用半自动、高产量 工艺单元，以优化材料使用，降低劳动力成本，确保液体和化学品回收，最终 导致制造成本大幅降低，从而加强了广泛的实施和 向更多地点提供治疗技术，特别是在贫困地区和农村地区 这些社区往往缺乏解决当地水污染问题的资源。 该项目的成果是在生物催化剂复合材料的多功能制造方面取得了重大进展 处理数百种污染物的核心。这一项目的成功结果意义重大 承诺解决使用现有技术无法有效处理的有害污染物，以及 从而在公共和私人管理水资源方面收回大量利益相关者的价值。

Project Summary / Abstract A variety of hazardous compounds continue to pose serious and widespread risks to public health and safety in the United States. Organic compounds, in particular, such as chlorinated solvents, hydrocarbons, disinfection byproducts, pesticides, polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and other xenobiotic (not naturally occurring) organic chemicals comprise a major category of water contaminants. These high-priority pollutants are often regulated by state and federal agencies due to their proven links to cancer and various diseases, including liver or kidney disease, immune dysfunction, nervous system disorders, and hormonal or reproductive defects. However, existing chemical, physical and biological technologies to remove these compounds suffer from significant limitations that lead to low treatment efficiency and/or increases costs for Superfund site managers, municipalities and water supplies. This project is based on a prior Phase II project involving the successful development of an enhanced cometabolism-based biological technology that significantly overcomes the limitations of conventional treatment systems. This new technology has been demonstrated to degrades the hazardous organic compounds into harmless byproducts instead of producing a concentrated secondary waste stream. Moreover, this new technology offers significant reductions in energy and maintenance costs compared with chemical or UV oxidation, and offers both an in-situ and ex-situ treatment options across a range of operating conditions to simultaneously and cost-effectively remove hazardous organic compounds from water. In this proposal, the manufacturing processes underlying this technology are significantly improved in terms of manufacturing throughput, waste reduction, quality control and environmental footprint. Specifically, the existing production process for the technology is redesigned and re-engineered using semi-automated, high-throughput process units to optimize material usage, reduce labor costs, ensure liquid and chemical recycling and ultimately lead to a significant reduction in manufacturing costs, thereby enhancing the widespread implementation and availability of the treatment technology to a greater number of sites, especially disadvantaged and rural communities which often lack the resources to address local water contaminations. The outcome of this project is a significant advance in the versatile manufacturing of biocatalyst composites at the heart of the treatment of hundreds of contaminants. The successful result of this project holds significant promise in addressing harmful contaminants that are not effectively treatable using existing technologies, and thereby recovering substantial stakeholder value for public and private stewardship of water resources.