DNA Repair Phenotype the Missing Link in Breast Cancer Risk Assessment

本申请是一份研究副刊，旨在促进与健康相关的研究的多样性，特别是 在哈泽姆·奥库诺拉博士培训的博士后研究阶段支持他，他的最终目标是 成为一名独立的、富有成效的环境健康研究人员。在本申请中1)父母 2)描述了奥库诺拉博士在家长赠款中拟议的活动，其中 将在保持其批准的具体目标的同时加强拨款中的研究；以及3)培训和 指导计划是专门为奥库诺拉博士量身定做的，旨在为他提供 是他成为一名独立的、多产的环境健康研究人员所必需的。 在Parent Grant的目标1A中，正在使用冷冻血液样本进行一项大型前瞻性队列研究 来自乳腺癌前瞻性家庭研究队列(Prof-SC)的个人，以评估其潜力 表型特征的DNA修复能力(DRC)与乳腺癌(BC)风险之间的关联。 奥库诺拉博士将负责对当前父母拨款的两项重大改进：1.超越 外周血单核细胞：探测外周血单核细胞亚型DNA修复能力的人与人之间的差异。总而言之，使用我们的 目前我们还不知道DNA修复能力的个体间差异是否统一源于 PBMC亚型或主要来源于特定的PBMC亚型。奥库诺拉博士提出的研究应该 允许对这个问题有一个明确的答案。2.展望未来：从血液样本中产生细胞系 这表明DNA修复能力要么减弱，要么增强。这里的目标是让奥库诺拉博士建立 DNA修复水平最高/最低的个体的PBMC样本中的永生化细胞系 容量。目前不存在由异常DRC定义的这种细胞系集合，预计它们 将成为我们团队和世界范围内未来基于机械的研究的宝贵资源。 本补编的关键方面将是培训和指导奥库诺拉博士成为一名独立的 富有成效的调查者，将在环境健康相关领域做出重大的长期贡献 生物医学研究。家长赠款是一个高度跨学科的项目，奥库诺拉博士将与 代表流行病学、DNA损伤/修复、生物标记物和生物统计学建模的学院。Dr。 奥库诺拉将参加一个定制的、有组织的博士后培训计划，重点是环境 卫生政策、专业精神、领导力和管理、沟通、职业管理和 负责任的研究行为。它将由多个不同的系列和微型课程组成，这些系列和迷你课程加在一起将 使他能够深入了解可用的职业发展选择，参与培训/教育 活动集中在他喜欢的职业道路上，坚持职业道德行为准则，磨练他的 领导/管理技能，以优化他在学术研究职位上的沟通技能，并 从训练过渡到独立。

This application is for a Research Supplement to promote diversity in health related research, specifically to support Dr. Hazeem Okunola in the postdoctoral research phase of his training, with the ultimate goal of his becoming an independent and productive environmental health researcher. In this application 1) The Parent Grant is briefly summarized; 2) Dr. Okunola's proposed activities within the Parent Grant are described, which will enhance the research in the grant while remaining within its approved Specific Aims; and 3) a Training and Mentoring Plan is described, specifically tailored for Dr. Okunola, designed to provide him with the skills that are needed for him to become an independent and productive environmental health researcher. In Aim 1A of the Parent Grant a large prospective cohort study is being undertaken using frozen blood samples from individuals from the Breast Cancer Prospective Family Study Cohort (ProF-SC), to assess the potential association between phenotypically characterized DNA Repair Capacity (DRC) and Breast Cancer (BC) risk. Dr. Okunola will be responsible for two significant enhancements of the current parent grant: 1. Beyond PBMCs: Probing person-to-person variations in DNA repair capacity in PBMC subtypes. In summary, using our current protocols we will not know if inter-individual differences in DNA repair capacity derive uniformly from all the PBMC subtypes or predominantly from specific PBMC subtypes. Dr. Okunola's proposed studies should allow a definitive answer to this question. 2. Looking to the Future: Generating cell lines from blood samples that show either diminished or enhanced DNA repair capacity. Here the goal is for Dr. Okunola to establish immortalized cell lines from PBMC samples from the individuals with the highest / lowest levels of DNA repair capacities. No such set of cell lines defined by anomalous DRC currently exist, and it is anticipated that they will become a valuable resource for future mechanistically based studies, both in our group and worldwide. The key aspect of this Supplement will be training and Mentoring Dr. Okunola to become an independent productive investigator who will make a significant long-term contribution in environmental-health-related biomedical research. The Parent Grant is a highly multidisciplinary project and Dr. Okunola will interact with faculty representing epidemiology, DNA damage / repair, biomarkers, and biostatistical modeling. Dr. Okunola's will participate in a customized structured postdoctoral training program focusing on environmental health policy, professionalism, leadership and management, communication, career management, and responsible conduct of research. It will consist of multiple distinct series and minicourses that together will enable him to gain insights into available career development options, to engage in training / education activities focused on his preferred career track, to adhere to professional ethical codes of conduct, to hone his leadership / managements skills, to optimize his communication skills for academic research positions, and to transition from training to independence.