Purchase of a Q-TOF LC/MS System

描述（由申请人提供）：要求资金从安捷伦技术公司购买一套精确质量Q-TOF LC/MS质谱仪系统。所要求的质谱仪系统（型号6520）的主要组件包括：配备双喷雾电喷雾电离（ESI）离子源的精确质量Q-TOF质谱仪，HPLC-Chip立方体质谱（MS）接口，纳流液相色谱（LC）系统；以及必要的数据系统和软件来获取、查看和处理质谱数据。所要求的仪器将被安置并维护在杜克大学化学系的仪器设施中，它不仅有助于满足本提案中概述的八个主要用户的一般质谱需求，而且还将有助于满足该设施整个用户群的一般质谱需求，其中包括化学系的20多名教职员工和化学系的100多名研究生和博士后研究员。以及杜克大学其他部门的研究人员（其中许多人参与了美国国立卫生研究院支持的研究项目）。该仪器将有助于分析各种样品类型，包括小有机分子以及合成和天然存在的生物聚合物。化学部门需要将Q-TOF质谱仪系统添加到其仪器设备中有三个方面的需求：(1)它将为该部门仪器设备中老化的主力机，16年的JEOL SX-102 HRMS系统提供替换能力；(2)它将减少与该部门的另一个主力质谱仪（已有7年历史的Agilent IonTrap LC-MS系统）相关的积压样品/用户；(3)它将为化学系仪器设施带来令人兴奋的新的蛋白质组学能力，这是校园内唯一一个为经过适当培训的用户提供直接步行访问其仪器的共享资源。所要求的仪器的可靠性、稳健性和易用性及其HPLC-Chip cube MS接口将极大地促进仪器在化学系设施开放访问环境中的操作。这将使该乐器成为杜克大学校园里独一无二的资源。

DESCRIPTION (provided by applicant): Funds are requested to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The major components of the requested mass spectrometer system (Model Number 6520) include: an accurate mass Q-TOF mass spectrometer equipped with a dual spray electrospray ionization (ESI) ion source, an HPLC-Chip cube mass spectrometry (MS) interface, a nanoflow liquid chromatography (LC) system; and the requisite data system and software to acquire, view, and manipulate mass spectral data. The requested instrument, which will be housed and maintained in the Duke Chemistry Department's Instrument Facility, will not only help meet the general mass spectrometry needs of the eight major users outlined in this proposal, but it will also help meet the general mass spectrometry needs of the Facility's entire user base, which includes 20+ faculty members in the Chemistry Department and 100+ graduate students and post-doctoral researchers in the Chemistry Department, as well as number of researchers from other departments at Duke (many of which are involved in NIH-supported research projects). The proposed instrument will facilitate the analysis of a wide variety of sample types, including small organic molecules as well as both synthetic and naturally occurring biopolymers. The Chemistry Department's need to add a Q-TOF mass spectrometer system to its Instrument Facility is three-fold: (1) it will provide replacement capabilities for an aging workhorse, the 16 year old JEOL SX-102 HRMS system, in the Department's Instrument Facility; (2) it will reduce the backlog of samples/users associated with another of the Department's workhorse mass spectrometers, a 7 year old Agilent IonTrap LC-MS system; and (3) it will bring exciting new proteomics capabilities to the Chemistry Department Instrument Facility, which is the only shared resource on campus to offer appropriately trained users direct walk-up access to its instruments. The reliability, robustness, and ease-of-use afforded by the requested instrument with its HPLC-Chip cube MS interface will greatly facilitate operation of the instrument in the Chemistry Department Facility's open access environment. This will make the instrument a unique resource on the Duke campus. PUBLIC HEALTH RELEVANCE: Funds are requested for the Duke University Chemistry Department Instrument Facility to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The instrument will be uniquely configured to facilitate the characterization of a wide variety of sample types ranging from small organic molecules to large naturally occurring biopolymers. The instrument will be available for direct walk-up use by appropriately trained users of the Duke Chemistry Department's Instrument Facility, many of whom are engaged in NIH-supported research.