TSE LabMaster - CaloS-Dri/Fed+AC/Act.XY-Tel, 3x8 Mice

描述（由申请人提供）：肥胖和相关疾病，如糖尿病，仍然是西方社会的主要健康威胁。为了更好地了解参与体脂调节的代谢过程的分子调节，需要沿着大量遗传和非遗传改变的动物模型以及越来越精细的一组工具来表征这些模型。我们需要一个24笼综合小鼠能量平衡分析系统。其主要特点是采用高速气体传感器的最先进的开路间接测热法，以及对食物和水的消耗、运动活动、能量消耗、身体核心温度和心率的同时和连续监测。重要的是，它在从冷暴露到动物热中性区（在那里它们需要最少的代谢能量来冷却或加热其生物体）的几个环境温度梯度内进行小鼠代谢表型分析。此外，该系统还包括一种通过智能软件“夹紧”食物摄入的新方法，以允许对细胞代谢和能量消耗进行独立的喂养研究。我们的建议的关键点是，所请求的系统将允许将小鼠模型的能量代谢分析作为这里列出的主要和次要用户的众多赠款和项目的中心部分，达到前所未有的技术复杂程度，这将允许高通量产生更具体、更详细、更相关和更科学和生理学上有意义的结果。直接相关的项目将从这一共享工具中受益匪浅，这些项目专注于通过CNS直接调节能量代谢、肠道激素-大脑相互作用或已知也调节能量代谢的炎症途径。最终，该仪器及其在辛辛那提大学正在进行的众多代谢和肥胖研究中的应用将促进对肥胖和糖尿病的更好理解。

DESCRIPTION (provided by applicant): Obesity and related disease such as diabetes remain major health threats in western societies. In order to better understand the molecular regulation of metabolic processes involved in body fat regulation, numerous genetically and pharmacologically altered animal models are necessary along with an increasingly refined set of tools to characterize these models. We are requesting a 24 cage Integrated Mouse Energy Balance Analysis system. Key features are state-of-the-art open-circuit indirect calorimetry with high-speed gas sensors, and simultaneous and continuous monitoring of food and water consumption, locomotor activity, energy expenditure, body core temperature and heart rate. Importantly, it performs mouse metabolic phenotyping within a gradient of several environmental temperatures from cold exposure to the animals thermoneutral zone (where they need the least possible metabolic energy to cool or heat their organism). Also, this system includes a novel way to "clamp" food intake by smart software to allow feeding independent study of cell metabolism and energy expenditure. The key point of our proposal is that the requested system will allow to take energy metabolism analysis of mouse models as center parts of numerous grants and projects by the here listed major and minor users to an unprecedented level of technical sophistication that will allow for high- throughput generation of more specific, more detailed, more relevant and more scientifically and physiologically meaningful results. Directly related projects which would massively benefit from this shared instrument are focusing on direct regulation of energy metabolism by the CNS, gut hormone - brain interactions or inflammatory pathways known to also regulated energy metabolism. Ultimately, this instrument and its use for the numerous ongoing metabolism and obesity studies at the University of Cincinnati will facilitate progress towards a better understanding of obesity and diabetes.