Acquisition Of A High-End, High-Speed Cell Sorter With Bio-Containment Hood

描述（由申请人提供）：我们提议获得一台18色、5激光BD Biosciences FACSAria-II细胞分选仪，配有生物防护罩和新型CellEvator声波动力样品混合器技术。该仪器将扩大研究人员在URMC流式细胞仪核心设施中获得细胞分选能力的可用性。自2008年4月收购我们目前的FACSAria-I和同时退役的老一代细胞分选机以来，我们的使用量从平均600小时/年（2008年4月之前）增加到一年内超过1200小时。 到2009年8月底，我们的预期使用增长将超过1500小时，我们的许多种类从中午12点运行到晚上8点。我们还处于一个位置，强调我们有能力从宝贵的患者样本中筛选出机会性临床样本。这种新的分拣机的增加将缓解这种担忧，使我们的转化研究计划取得进一步进展。 FCC与罗切斯特人类免疫学中心（RHIC）密切合作，在中期开发了信息丰富的分析流程板，（8-12色）和高端（>12色）区域，在研究B细胞在自身免疫性疾病中的作用方面产生了越来越重要，更相关，更全面的数据，改善淋巴瘤治疗的措施，研究造血微环境，B和T细胞对病原体（包括线虫和流感）的免疫反应，以及早期发育模型中造血干细胞的正常成熟。 有了识别和划分复杂群体的能力，就需要隔离和研究这些子集。因此，所提出的仪器将增强和扩展我们的能力，从仅仅识别和计数这些细胞，到能够分离和研究非常详细的工具，可用于正常和疾病状态的基因组，蛋白质组学和生物化学表征。 电子学、激光器和光学的系统性进步使得当前的仪器能够从单个细胞中同时检测多达18个荧光参数。这使得通过高端仪器检测更多的荧光标记物成为绝对必要的，以捕捉细胞活性的每一个细微差别，揭示正常细胞和病变细胞之间最细微的差异-特别是对于罕见患者样本的临床转化研究。

DESCRIPTION (provided by applicant): We propose to acquire an 18-color, 5-laser BD Biosciences FACSAria-II cell sorter, with bio- containment hood and novel CellEvator acoustic wave powered sample mixer technology. This instrument will expand the availability of researchers to access cell sorting capabilities in the URMC Flow Cytometry Core facility. Since the acquisition of our current FACSAria-I, in April of 2008 and concurrent retirement of an older generation cell sorter, our usage has gone from an average of 600 hours/year (pre April, 2008), to over 1200 hours in one year. Our anticipated growth in usage will top over 1500 hours by the end of August, 2009, with many of our sorts running from 12 noon till 8 pm. We are also in a position that stresses our ability to sort opportunistic clinical samples from precious patient samples. The addition of this new sorter will alleviate this concern, allowing further advances in our translational research programs. The FCC, in close collaboration with the Rochester Human Immunology Center (RHIC), has been very successful developing informative analytical flow panels in the mid- (8-12 color) and high end (>12 color) area, which has generated increasingly important, more relevant, and comprehensive data in the study of the role of B cells in autoimmune diseases, measures to improve treatment of lymphoma, studies of the hematopoietic microenvironment, B and T cell immune responses to pathogens including nematodes and influenza, and normal maturation of the hematopoietic stem cell in early developmental models among other efforts. With the ability to identify and sub-set complex populations, comes the need to isolate and study these sub-sets. Thus the proposed instrument will augment and extend our abilities from just identification and enumeration of these cells, to the ability to isolate and study in great detail with the host of tools available for genomic, proteomic and biochemical characterization of normal and diseased states. Systematic advances in electronics, lasers, and optics have made current instruments capable of detection of up to 18 simultaneous fluorescent parameters from a single cell. This makes the detection of more fluorescent markers by high-end instrumentation an absolute necessity in order to capture every nuance of cell activity to reveal the subtlest differences between normal and diseased cells - most especially for clinical-translational research on rare patient samples.