Core--Genomics

描述（由申请方提供）：土拉热弗朗西斯菌是一种高度传染性病原体，可通过气溶胶途径获得。因此，它已被列为A类 由CDC选择代理。没有许可的疫苗可用于预防这种感染。 有机体基因组学方法在很大程度上依赖于微阵列的开发和分析，将是解开有关生物体毒力以及宿主在感染期间的反应之谜的关键。该核心将侧重于支持本PO 1应用程序中的所有项目，目标如下：目标1是建造和利用F。土拉热菌Schu 4寡核苷酸微阵列;以及，目标2是对DNA测序、寡核苷酸生产和其他核酸检测/分析功能（例如，真实的时间PCR和Typhoon成像系统）。在该核心的支持下，各个项目将导致弗朗西斯菌发病机制和免疫领域的新发现。

DESCRIPTION (provided by applicant): Francisella tularensis is a highly infectious pathogen that can be acquired by the aerosol route. As such, it has been classified as a Category A select agent by the CDC. No licensed vaccine is available for protection against infection by this organism. Genomic approaches, relying heavily on microarray development and analysis, will be key to unlocking the mysteries concerning the virulence of the organism, as well as the response of the host, during infection. This Core will be focused on support for all of the Projects in this PO1 application with the following aims: Aim 1 is construction and utilization of a F. tularensis Schu4 oligonucleotide microarray; and, Aim 2 is support services to the program project for DNA sequencing, oligonucleotide production, and other nucleic acid detection/analytical functions (e.g., Real Time PCR and Typhoon Imaging system). With the support of this Core, the individual projects will lead to novel discoveries in the fields of Francisella pathogenesis and immunity.