Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project

项目总结/摘要 生食中肠道菌群的检测及耐药性监测 爱荷华州零售肉类和海鲜：NARMS零售食品监督项目 抗生素耐药性（AMR）是影响人类健康的主要健康威胁。 人类、动物和世界各地的环境。强大而全面的监控 强调“同一健康”方法的系统对于有效对抗AMR是必要的。 在美国，国家抗菌素耐药性监测系统的零售部门 美国食品药品监督管理局（FDA）下属的NARMS、兽医中心（FDA- CVM）在选定的肠道细菌（非伤寒沙门氏菌， 弯曲杆菌，肠球菌和大肠杆菌）从零售生肉（鸡肉，地面 火鸡和牛肉，以及碎猪肉/排骨）。最近，零售业中的AMR监测 海产品（罗非鱼、虾、鲑鱼）中选择细菌（弧菌、气单胞菌、大肠杆菌）。大肠杆菌，肠球菌， 产碳青霉烯酶肠杆菌属）也已在至少8个州启动。的 NARMS计划为监测AMR的来源和趋势做出了重大贡献， 食物链为了加强打击AMR的努力，FDA发起了一个合作项目， 该协议计划将于2016年在美国全境扩大NARMS监视网络。 在该倡议下，爱荷华州州立大学（ISU）于2004年年底成为NARMS网络实验室。 2016.自那时以来，执行支助股网站为加强能力和 全国零售食品AMR监测计划的有效性。在本申请中，我们 建议继续与NARMS建立合作伙伴关系，以实现 FOA PAR-20-124“NARMS Cooperative”中所述的零售食品监督计划 加强零售食品抗生素耐药性监测的协议计划 标本”。具体来说，我们会继续收集和处理生肉样本和海产品 从爱荷华州的杂货店收集样品并培养它们以分离感兴趣的细菌， 由NARMS定义，并每月将分离株运送给FDA。此外，我们将 继续进行沙门氏菌、弯曲杆菌和大肠杆菌的全基因组测序。杆菌 从肉类样本中分离出的病毒，并通过GenomeTrakr与FDA共享序列。这 应用程序是建立在现有的经验，是一个自然的延伸，我们目前的努力， 纳姆斯。成功完成联合国难民事务高级专员办事处工作所需的训练有素的人员和后勤基础设施 计划的工作已经到位。我们坚信，执行支助股继续参与这项工作， 合作网络将大大有助于缓解食物链中的AMR， 最终提高食物安全和公众健康。

Project Summary/Abstract Detection and surveillance of antimicrobial resistance among enteric bacteria from raw retail meat and seafood in Iowa: A NARMS Retail Food Surveillance Project Antimicrobial resistance (AMR) is a major health threat affecting the well-being of humans, animals, and the environment worldwide. Robust and comprehensive surveillance systems emphasizing the “One Health” approach are necessary for effectively combatting AMR. In the United States, the retail arm of the National Antimicrobial Resistance Monitoring System (NARMS) under the Food and Drug Administration, Center for Veterinary Medicine (FDA- CVM) performs surveillance of AMR in select enteric bacteria (non-typhoidal Salmonella, Campylobacter, Enterococcus and Escherichia coli) from retail raw meats (chicken, ground turkey and beef, and ground/chop pork) in 18 states. Recently, surveillance of AMR in retail seafood (tilapia, shrimp, salmon) for select bacteria (Vibrio, Aeromonas, E. coli, Enterococcus, carbapenemase-producing Enterobacteriaciae) has also been started in at least 8 states. The NARMS program has contributed significantly to monitoring the sources and trends of AMR in the food chain. To enhance the effort on combating AMR, FDA launched a cooperative agreement program to expand the NARMS surveillance network throughout the U.S in 2016. Under the initiative, Iowa State University (ISU) became a NARMS network laboratory in late 2016. Since then, the ISU site has contributed substantially to strengthening the capacity and effectiveness of the national AMR surveillance program in retail food. In this application, we propose to continue the collaborative partnership with NARMS to address the updated goals of the Retail Food Surveillance Program as described in FOA PAR-20-124 “NARMS Cooperative Agreement Program to Strengthen Antibiotic Resistance Surveillance in Retail Food Specimens”. Specifically, we will continue to collect and process raw meat samples and seafood samples from grocery stores in Iowa and culture them for isolation of bacteria of interest as defined by NARMS, and ship the isolates to FDA on a monthly basis. In addition, we will continue to perform whole-genome sequencing of Salmonella, Campylobacter and E. coli isolates from the meat samples and share the sequences with FDA via GenomeTrakr. This application is built on existing experience and is a natural extension of our current effort with NARMS. Trained personnel and logistic infrastructure required for successful completion of the planned work are already in place. We strongly believe that ISU’s continued participation in this collaborative network will contribute significantly to mitigation of AMR in the food chain and ultimately to enhancement of food safety and public health.