Core C: Pathology, Biospecimen and Immune Profiling

病理学、生物医学和免疫分析核心将选择、存储和提供集中的 对收集在应答队列中的生物检疫进行病理分析，确保一致的高质量 所有下游分析均可使用生物素分析物。核心将主要支持项目2， 3和4有生物标本、生物标本分析物以及病理和图像数据库。主要功能 核心是病理注释和福尔马林固定石蜡包埋(FFPE)前列腺癌 响应内约3,000名患者的组织(项目2、3和4)，分离和量化细胞核 从这些组织(项目3)和7,500个唾液样本(项目2)中提取适当质量的酸 控制/质量保证措施，并测试和实施审讯图像分析技术 肿瘤相关免疫原位浸润物(项目4)。中心的活动将集中在约翰·霍普金斯大学 大学(JHU)，并将利用众多正在进行的生物谱库努力和前列腺癌 JHU的组织分析，包括JHU前列腺癌孢子生物显微镜/病理核心和 CDMRP前列腺癌生物信息库网络(PCBN)。核心将由两名泌尿科病理学家领导， Tamara Lotan医学博士(Pi，JHU)和Angelo M.de Marzo医学博士(co-I，JHU)以及两名科学家 具有与核心、Karen Sfan os博士(co-I、JHU)相关的特定生物谱系分析的丰富专业知识 克里斯·海曼(Co-I，南加州大学)。除了为3个项目提供广泛支持外，核心的生物库和数据库将 为所有未来的研究工作提供持续的资源，利用响应队列和其 生物群落。

The Pathology, Biospecimen and Immune Profiling Core will select, bank, and provide centralized pathologic analysis for biospecimens collected in the RESPOND cohort, ensuring that uniformly high quality biospecimen analytes are available for all downstream analyses. The Core will principally support Projects 2, 3 and 4 with biospecimens, biospecimen analytes and pathologic and image databases. The major functions of the Core are to pathologically annotate and bank formalin fixed paraffin embedded (FFPE) prostate tumor tissue for approximately 3,000 patients within RESPOND (Projects 2, 3 and 4), isolate and quantify nucleic acids from these tissues (Project 3) and from 7,500 saliva samples (Project 2) with appropriate quality control/quality assurance measures, and test and implement image analysis technologies for interrogating tumor-associated immune infiltrates in situ (Project 4). The Core’s activities will be centered at Johns Hopkins University (JHU) and will leverage the numerous ongoing biospecimen banking efforts and prostate tumor tissue analyses at JHU, including the JHU Prostate Cancer SPORE Biospecimen/Pathology Core and the CDMRP Prostate Cancer Biorepository Network (PCBN). The core will be led by two urologic pathologists, Tamara Lotan MD (PI, JHU) and Angelo M. De Marzo MD PhD (co-I, JHU) as well as two scientists with extensive expertise for specific biospecimen analyses relevant to the Core, Karen Sfanos PhD (co-I, JHU) and Chris Haiman (co-I, USC). In addition to extensive support for 3 Projects, the Core’s biobank and database will provide an ongoing resource for all future research endeavors utilizing the RESPOND cohort and its biospecimens.