Development of an integrated smartphone/resistive nanosensor for onsite biomonitoring of exposure to pesticides

项目摘要 有机磷农药中毒是一个世界性的重大环境和职业健康问题。 由于其急性或慢性毒性。快速准确地检测个人接触有机磷农药是 对受害者的研究和临床护理都至关重要。然而，这种检测仍然具有很高的可变性 商业上可用的检测方法，例如Ellman方法，并且这种检测方法也可能不能充分检测Low 但与临床相关的暴露生物标志物水平。此外，这些化验通常在 集中式实验室，速度慢，成本高。这些赤字转化为错失了 及时开始对急性或慢性中毒受害者进行最适当的治疗和干预。 一种简单、快速、非或微创的生物监测工具，用于现场灵敏和准确地检测 个人接触有机磷农药是非常可取的，符合NIEHS暴露计划的兴趣。这 STTR第一阶段项目是开发创新的集成智能手机/纳米传感器平台，用于现场快速和 使用一小滴手指棒血灵敏地检测OP暴露。这种纳米传感器将利用 纳米技术和无线技术，并利用重要的生物标记物，血液胆碱酯酶进行检测 和由此产生的工具有可能成为环境和环境卫生的mHealth 职业工人。研究将确定检测下限、灵敏度、动态范围等关键参数 使用添加典型农药的体外血液样本的传感平台性能指标 使用农场工人的指棒血样在野外验证了平台，并论证了其可行性 灵敏而准确地检测接触有机磷农药的平台。由此产生的纳米传感器 将是一种简单、快速、成本效益高、侵入性最小的生物监测工具，可以准确地 个人暴露评估和现场快速检测有机磷农药暴露。因此，这部小说 传感平台可以作为个人自检仪表，就像血糖仪一样，也可以用于 现场、医生办公室、床边和急诊室。

Project Summary Organophosphate (OP) pesticides poisoning is a major environmental and occupational health problem worldwide due to its acute or chronic toxicity. Rapid and accurate detection of personal exposure to OP pesticides is critical to both research and clinical care for victims. However, this detection remains highly variable across commercially available assays, e.g., the Ellman method, and such assays may also not adequately detect low but clinically relevant levels of exposure biomarkers. Moreover, these assays are generally performed in the centralized laboratories, and is slow and expensive. These deficits translate into missed opportunities for the timely initiation of the most appropriate treatment and interventions for acutely or chronically poisoned victims. A simple, rapid, non- or minimally invasive biomonitoring tool for onsite sensitive and accurate detection of personal exposure to OP pesticides is highly desirable and fits the interest of NIEHS exposure program. This STTR phase I project is to develop an innovative integrated smartphone/nanosensor platform for onsite rapid and sensitive detection of OP exposure using a tiny drop of finger-stick blood. This nanosensor will take advantage of nanotechnology and wireless technology and utilize an important biomarker, blood cholinesterase, for the detection of personal OP exposure and the resulted tool has the potential to serve as mHealth for environmental and occupational workers. The research will determine the detection limit, sensitivity, dynamic range, and other key performance metrics of the sensing platform using blood samples in vitro dosed with typical pesticides and validate the platform in field using finger-stick blood samples from farmworkers, and demonstrate the feasibility of the platform for sensitive and accurate detection of exposure to OP pesticides. The resulted nanosensor would be a simple, rapid, cost-effective, and minimally-invasive, point-of-care biomonitoring tool for accurate personal exposure assessment and for on-site rapid detection of exposure to OP pesticides. Thus, this novel sensing platform could serve as a personal meter for self-test just like a glucose meter and could also be used in field, physician's office, bedside, and emergency room.