Core 2: Heterogeneity of Aging

项目摘要-老化核心的异质性 与Nathan Shock的开创性工作一致，很明显，老化的组织积累了细胞异质性 或者是马赛克。这种异质性很可能是由于细胞间相互作用受损而导致衰老的一个原因。 和组织功能的协调。SAN内老化核心(异质核心)的异构性 迭戈·内森老年基础生物学卓越中心(SD-NSC)将使研究人员能够 探索大范围(从分子到细胞器再到单个细胞)衰老的异质性 组织)通过提供对各种最先进的仪器和分析技术的访问， 以及经验丰富的核心员工。核心将为研究关键过程提供具体资源 与衰老和疾病有关的高分辨率，包括单细胞下一代测序平台， 测量蛋白质组和代谢组学的高分辨率成像系统和质谱学方法 细胞和组织中衰老的特征，重点是细胞-细胞异质性和跨 纸巾。这些技术正在迅速发展，并将在未来几年继续发展。利用 拥有与不断发展的技术保持同步的可靠跟踪记录的成熟核心资源是最多的 确保分析技术的新创新最大限度地应用于 年迈的研究人员。异质核心通过以下方式为老龄化领域的研究人员提供具体支持：1) 提供获得特定科学服务、建议和专业知识的途径；2)开发和传播 相关数据的获取方法；3)开展现场培训和虚拟培训。这个 异质性核心是我们的SD-NSC将创建的研究资源管道中的关键组件。 这一核心的价值得到了与年龄相当的诱导细胞类型和器官的产生的支持 老龄化核心的人体细胞模型和老龄化综合模型中的新型机器学习能力 核心。我们将共同为衰老基础生物学领域的研究人员提供必要的资源 对我们变老的机制做出关键发现。异质核心将使研究能够 衰老的细胞-细胞和组织的异质性，以及最终的贡献和机制 异质性导致退化和功能障碍，这是衰老的特征。

PROJECT SUMMARY – Heterogeneity of Aging Core In line with the pioneering work of Nathan Shock, it is clear that aged tissues accumulate cellular heterogeneity or mosaicism. This heterogeneity is likely a cause of aging due to impairments in both intercellular interactions and the coordination of tissue function. The Heterogeneity of Aging Core (Heterogeneity Core) within the San Diego Nathan Shock Center of Excellence in Basic Biology of Aging (SD-NSC) will enable investigators to probe the heterogeneity of aging over a broad range of scales (from molecules to organelles to single cells and tissues) by providing access to a diverse suite of state-of-the-art instrumentation and analytical technologies, as well as experienced Core staff. The Core will provide specific resources for studying key processes implicated in aging and disease at high resolution, including single-cell next-generation sequencing platforms, high-resolution imaging systems, and mass spectrometry approaches to measure proteomic and metabolomics signatures of aging in cells and tissues, with an emphasis on cell-cell heterogeneity and heterogeneity across tissues. These technologies are rapidly evolving and will continue to do so over the coming years. Utilizing established Core resources with proven track records for staying current with evolving technologies is the most effective way to ensure new innovations in analytical technologies are available to the greatest breadth of aging researchers. The Heterogeneity Core provides specific support for researchers in the aging field by: 1) providing access to specific scientific services, advice, and expertise, 2) developing and disseminating novel methods for correlative data acquisitions, and 3) running on-site and virtual training sessions. The Heterogeneity Core is a critical component in the pipeline of research resources that our SD-NSC will create. The value of this Core is bolstered by the generation of age-equivalent induced cell types and organoids by the Human Cell Models of Aging Core, and novel machine-learning capabilities in the Integrative Models of Aging Core. Together we will provide researchers in the basic biology of aging field with the resources necessary to make key discoveries into the mechanisms by which we age. The Heterogeneity Core will enable studies into the cell-cell and tissue heterogeneity of aging and, ultimately, the contributions and mechanisms by which heterogeneity causes the degeneration and dysfunction that characterizes aging.