课题基金 / 基金详情

Disrupting Type IV secretion function to prevent virulence in the pathogenic bacterium Legionella pneumophila

Disrupting Type IV secretion function to prevent virulence in the pathogenic bacterium Legionella pneumophila
破坏 IV 型分泌功能以防止致病菌嗜肺军团菌的毒力
批准号：
10269713
负责人：
Anton Simeonov
金额：
$18.66万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10269713
关键词：
Alveolar MacrophagesAmoeba genusBiological AssayCellsCoxiellaEnvironmentFresh WaterGrowthImmune responseInfectionLegionella pneumophilaLegionnaires&apos DiseasePneumoniaProcessProteinsReporterSignal TransductionSystemToxinType IV Secretion System PathwayValidationVirulencedesignnovel therapeuticspathogenpathogenic bacteriapreventscreeningsmall moleculetrafficking

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相关文献

中文摘要
翻译
在此期间，项目团队设计了一种新的筛选方法，该方法强烈支持通过直接靶向细菌T4SS而不是宿主细胞内的过程来鉴定干扰报告易位过程的化合物。成功完成了对数万个分子的初步筛选，并对命中分子进行了精心挑选和重新测试以进行验证。进一步评估选择的命中物以确定它们在需要功能性T4SS的多种生物测定中的功效，例如在伯内特考克斯氏菌中。
英文摘要
During this period, the project team designed a new screening approach that strongly favored identification of compounds that interfere with the process of reporter translocation by directly targeting the bacterial T4SS rather than processes within host cells. Primary screening of tens of thousands of molecules was successfully completed, and hit molecules were cherry-picked and retested for validation. Select hits were further assessed to determine their efficacies in a variety of biological assays that require a functional T4SS, for example in Coxiella Burnetti.
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