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Activation-induced cytidine deaminase

Activation-induced cytidine deaminase
激活诱导的胞苷脱氨酶
批准号：
10011394
负责人：
Anton Simeonov
金额：
$25.03万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10011394
关键词：
AntibodiesB-Cell ActivationBiological AssayBiological ProcessCytosineCytosine deaminaseDNADNA Double Strand BreakDNA RepairDeaminationDevelopmentDrug resistanceFluorescence Resonance Energy TransferGenomeIGH@ gene clusterImmunoglobulin Class SwitchingImmunoglobulin Somatic HypermutationImmunoglobulin Switch RecombinationImmunoglobulin Variable RegionIn VitroMusMutagenesisMutationPlayPoint MutationRoleSplenocyteStretchingUracilWorkactivation-induced cytidine deaminaseanti-cancer therapeuticbasecancer cellinhibitor/antagonistnovelscreeningsmall molecule librariestumor initiation

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中文摘要
翻译
该项目旨在确定新的AID抑制剂作为潜在的抗癌治疗剂。 项目团队打算使用基于FRET的胞嘧啶脱氨基分析筛选NCGC小分子文库，以鉴定AID催化活性的潜在抑制剂。将在体外鼠B细胞活化试验中检查鉴定的命中，以确认生物学功能，并将在野生型原代脾细胞中进一步表征选定的经验证的命中分子，以鉴定在适当范围内具有抑制效力的分子。 在此期间，该项目团队致力于概念化和优化高通量适用测定，以筛选NCGC的化学文库。
英文摘要
This project aims to identify novel AID inhibitors as potential anticancer therapeutics. The project team intends to screen NCGC small molecule libraries using FRET-based analysis of cytosine deamination to identify potential inhibitors of AID catalytic activity. Identified hits will be examined in an in vitro murine B-cell activation assay to confirm biological function, and select validated hit molecules will be further characterized in wild type primary splenocytes to identify molecules with inhibitory efficacy in the appropriate range. During this period, the project team worked to conceptualize and optimize a high-throughput amenable assay to enable screening of NCGC's chemical libraries.
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