Core B - Biomarkers Core

项目总结/摘要-核心B： 生物标志物核心（核心B）的目标是通过以下方式支持本P01申请中的项目1-3和核心C 为广泛的分析和生物化学测定提供生物分析支持， O2-/H2 O2的产生、氢过氧化物代谢和氧化还原过程的基本氧化还原生物学 与临床前模型中药理学抗坏血酸盐（P-AscH-）研究相关的活性金属离子， 临床试验核心B将提供经验证的试剂，例如关键试剂的储备液、标准品， 海马和EPR分析、酶学分析、电离辐射服务和分子生物学 试剂，项目1-3。例如，我们独特、高效且具有成本效益的测定方法， 患者源性样本中的血浆抗坏血酸盐浓度（项目1和项目2）以及 在建议的临床前研究中产生的各种样品中的抗坏血酸（项目1-3）。核心B 有一个大的剧目的测定，将检查基本的氧化还原生物学的P-AscH-。核心的领导者 我们还开发了新的独特的分析和方法，专注于解决核心问题， 本P01中拟定的临床试验。领导者在该领域拥有30多年的经验， 在指导爱荷华州的卡弗医学院和霍尔顿综合核心设施的大学 癌症中心因此，他们完全有资格领导这个核心。

Project Summary/Abstract - Core B: The goal of the Biomarkers Core (Core B) is to support Projects 1-3 and Core C in this P01 application by providing bioanalytical support for a broad range of analytical and biochemical assays that address the fundamental redox biology of the production of O2-/H2O2, hydroperoxide metabolism, and processing of redox active metal ions relevant to the study of pharmacological ascorbate (P-AscH-) in preclinical models and clinical trials. Core B will provide validated reagents, e.g. stock solutions of key reagents, standards, Seahorse and EPR analyses, enzymological analyses, ionizing radiation services, and molecular biology reagents, to Projects 1-3. Examples range from our unique, efficient, and cost-effective assays for determining plasma ascorbate concentrations in patient-derived samples (Project 1 and Project 2) as well as assays for ascorbate in the wide variety of samples generated in the proposed preclinical studies (Projects 1-3). Core B has a large repertoire of assays that will examine the basic redox biology of P-AscH-. The leaders of the core have also developed new unique assays and methods that are focused on addressing questions central to the proposed clinical trials in this P01. The leaders have more than 30 years of experience in this field as well as in directing University of Iowa core facilities in the Carver College of Medicine and the Holden Comprehensive Cancer Center. Thus, they are well qualified to lead this core.