Core 2: Animal Models

项目摘要--核心2 核心2(动物模型核心)的总体目标是支持动物模型的开发和使用 在本计划项目赠款(PPG)中使用。动物模型核心的指导原则是有效的 动物的规划和利用，加工和诊断的标准化，服务的可靠性和 对动物模型的一致评估。核心内提供的基本专业知识是实验动物 医学、临床和解剖病理学。核心部门的办公和协调工作设在 兽医生物科学。Stefan Niewiesk博士，被提名为核心主任一直担任动物核心主任 自2004年以来，他是PPG所有主要调查人员的积极合作者。Niewiesk博士是一个 获得兽医微生物学和实验动物医学证书(欧洲学院)。克里斯塔·拉·珀尔博士 (联合调查员)，谁是由美国兽医病理学院认证的将提供病理 对核心的评估。PPG的总主题是分析感染HTLV-1是如何导致 CD4T细胞的增殖，病毒潜伏期和蛋白质如何影响和维持转化，以及 肿瘤细胞与其微环境相互作用。在急性感染后，HTLV-1在机体内持续存在， 最终导致CD4T细胞的寡克隆转化和生长。成立后及 病毒潜伏期的重新激活(项目3：Ratner)，肿瘤的发生是由病毒蛋白Hbz驱动的(项目1： 绿色)。这些白血病细胞扩散到整个生物体并引起骨溶解(项目2：Weilbaecher)。 所有项目都将使用兔和小鼠的成人T细胞白血病模型，这些模型已经建立和/或 核心2使用HTLV-1的分子克隆。

PROJECT SUMMARY – CORE 2 The overall goal of Core 2 (Animal Model Core) is to support the development and use of the animal models utilized within this Program Project Grant (PPG). The guiding principles for the animal model core are efficient planning and utilization of animals, standardization of processing and diagnoses, reliability of service and consistent evaluation of animal models. The fundamental expertise provided within the core is laboratory animal medicine and clinical and anatomic pathology. The Core is housed and coordinated in the Department of Veterinary Biosciences. Dr. Stefan Niewiesk, proposed Core Director has been Director of the Animal Core since 2004 and is an active collaborator with all of the principal investigators for the PPG. Dr. Niewiesk is a certified in Veterinary Microbiology and in Laboratory Animal Medicine (European College). Dr. Krista La Perle (Co-Investigator), who is certified by the American College of Veterinary Pathology will provide pathological evaluations for the Core. The overall theme of the PPG is to analyze how infection with HTLV-1 induces proliferation of CD4 T cells, how viral latency and proteins influence and maintain transformation and how the tumor cells interact with their microenvironment. After acute infection, HTLV-1 persists in the organism, eventually leading to oligoclonal transformation and outgrowth of CD4 T cells. After establishment and reactivation of viral latency (Project 3: Ratner), tumorigenesis is driven by the viral protein Hbz (Project 1: Green). These leukemic cells spread throughout the organism and cause osteolysis (Project 2: Weilbaecher). All projects will use rabbit and mouse models for adult T cell leukemia, which have been established and/or utilized by Core 2 using a molecular clone of HTLV-1.