Animal Shared Resource

项目摘要-动物共享资源 Wistar癌症中心运营着AAALAC认可的动物共享资源(ASR)服务教职员工 来自所有三个科学项目。专家管理、畜牧、看管和兽医工作人员维护 ASR，包括修改后的生物安全二级(BSL2)屏障，具体目标如下： ·提供实验动物医药和畜牧业、日常动物采购、动物保健服务 监测、兽医护理、库存监测、隔离住房、例行技术支持和 为所有癌症中心研究人员提供技术培训，在他们的研究中使用动物。 ·保持NSG(NOD.Cg-Prkdccid Il2rgtm1Wjl/Szj)繁殖群体，以产生免疫缺陷 老鼠。 ·通过我们的相互合作，为转基因和转基因小鼠的产生提供专业知识 与福克斯·蔡斯癌症中心的转基因小鼠机构合作。 ·提供全职兽医病理学家大卫·加里克的服务。 ·管理机构动物保护和使用委员会(IACUC)要求新员工进行培训，并 IACUC程序培训以及新研究人员的无菌技术培训。 ·拥有活体成像套件，包括PerkinElmer IVIS Spectrum？CT成像系统。 建造完成，新的，最先进的间隔室于2013年11月开放。配备有 机架空间可容纳6000多个无菌、一次性和单独通风的鼠笼，该品牌 新的，13,570 NSF，vivarum使用技术先进的一次性和单独通风的笼子 由新的暖通空调系统支持。自2013年以来，已有超过1400万美元的机构投资 分配给ASR，以完成癌症中心(CC)的使命和研究目标。这一点意义重大 资本分配允许建造和安装新的隔室，包括增强 为癌症中心研究人员提供动物护理的所有领域。访问控制包括身份证刷卡、生物识别 指纹扫描和安全摄像头。休息室配备了新的通风架子。进一步 投资包括隔离隔间，它是独立于物种的，并允许最大限度的灵活性，而 提供了遏制和隔离的行业标准。在项目期间，招聘了10名 CC的新成员推动了小鼠动物模型的使用增加了47%。一只额外的动物 为满足日益增长的服务需求，2016年聘请了CATAKER。简化日常运营 在ASR中，新的IACUC协议和动物设施管理系统将于2018年纳入。 总之，ASR将继续发挥重要作用，支持所有三个项目的科学组合 癌症中心，对关键的多学科科学进步做出了切实的贡献。

PROJECT SUMMARY – ANIMAL SHARED RESOURCE The Wistar Cancer Center operates an AAALAC—accredited Animal Shared Resource (ASR) serving faculty from all three scientific Programs. Expert management, husbandry, custodial and veterinary staff maintain the ASR, including a modified Biosafety Level 2 (BSL2) barrier, with the following specific aims: · Provide service in laboratory animal medicine and husbandry, routine animal procurement, animal health surveillance, veterinary care, inventory monitoring, quarantine housing, routine technical support and technical training for all Cancer Center investigators using animals in their research. · Maintain an NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) breeding colony for the generation of immunodeficient mice. · Provide access to expertise for the generation of transgenic and genetically modified mice through our inter- institutional collaboration with the transgenic mouse facility at Fox Chase Cancer Center. · Provide access to the services of a full time veterinary pathologist, David Garlick, D.V.M. · Administer Institutional Animal Care and Use Committee (IACUC) mandated new employee orientation and IACUC procedures training as well as aseptic technique training for new researchers. · House a suite for in vivo imaging, including a PerkinElmer IVIS Spectrum µCT Imaging System. Construction was completed, and the new, state-of-the-art vivarium opened in November 2013. Equipped with rack space to house more than 6,000 sterile, disposable, and individually-ventilated mouse cages, the brand new, 13,570 NSF, vivarium employs technologically-advanced, disposable, and individually-ventilated cages supported by a new HVAC system. Since 2013, an institutional investment of more than $14 million has been allocated to the ASR to fulfill the mission and research objectives of the Cancer Center (CC). This significant capital allocation allowed for the construction and fit-out of the new vivarium, including features which enhance all areas of animal care for Cancer Center investigators. Access controls include ID card swipe, biometric fingerprint scan, and security cameras. Holding rooms are equipped with new ventilated racks. Further investments include isolation cubicles, which are species independent and allow for maximum flexibility while providing the industry standard for containment and isolation. During the project period, the recruitment of 10 new CC members has driven a 47% increase in the use of murine animal models. An additional animal caretaker was hired in 2016 to meet the increasing demand for services. To streamline day-to-day operations of the ASR, a new IACUC protocol and Animal Facility Management system is being incorporated in 2018. Together, the ASR continues to play an essential role to support the scientific portfolio of all three Programs in the Cancer Center, tangibly contributing to key multidisciplinary scientific advances.