Phenotyping Core

耶鲁大学MPMOD表型核心的总体目标是为校外调查人员提供访问 耶鲁大学MPMOD以公平合理的价格提供独特的代谢表型服务，以及 使他们能够描述他们的糖尿病和肥胖症基因操纵小鼠模型的影响 关键代谢参数及全身和组织特异性细胞内代谢通量的研究 最先进的稳定和放射性标记的同位素方法学。耶鲁大学MPMOD表型的具体目标 核心是：1)为科学界提供标准化、高质量、独特的代谢途径 耶鲁大学MPMOD目前以公平合理的价格提供的表型鉴定服务，2)继续 建立新的稳定同位素GC-MS、LC-MS/MS和核磁共振方法，用于全身代谢表型和 清醒小鼠的细胞内代谢，以及3)参与并与 MPMOD和MPMOD协调股在#年维持代谢表型数据的国家数据库 肥胖和糖尿病的小鼠模型。预计耶鲁大学MPMOD产生的结果将领先于 为了更好地了解糖尿病和肥胖症的病理生理学及其相关代谢 并发症并导致新的治疗方法。最终，来自体内代谢表型的洞察力 将有助于开发针对新陈代谢的精确药物方法 病理生理学及其下游并发症。

The Overarching Aim of the Yale MPMOD Phenotyping Core is to provide extramural investigators access to the unique metabolic phenotyping services provided by the Yale MPMOD at a fair and reasonable price, and empower them to characterize the impact of their genetically manipulated mouse models of diabetes and obesity on key metabolic parameters and whole body and tissue specific intracellular metabolic fluxes using state-of- the-art stable and radio-labeled isotope methodology. The Specific Aims of the Yale MPMOD Phenotyping Core are: 1) To provide the scientific community access to standardized, high quality, unique metabolic phenotyping services currently available at the Yale MPMOD at a fair and reasonable cost, 2) To continue to develop novel stable isotope GC-MS, LC-MS/MS and NMR methods to metabolically phenotype whole body and intracellular metabolism in awake mice, and 3) To participate and collaborate with the National Consortium of MPMODs and the MPMOD Coordinating Unit to maintain a national database of metabolic phenotyping data in mouse models of obesity and diabetes. It is anticipated that the results generated by the Yale MPMOD will lead to a better understanding of the pathophysiology of diabetes and obesity and their related metabolic complications and lead to new therapies. Ultimately, the insights generated from in vivo metabolic phenotyping in mouse models will facilitate the development of precision medicine approaches targeting metabolic pathophysiology and its downstream complications.