3D optical histopathology for head and neck intraoperative consultation.

项目摘要 术中会诊(IOC，通常指手术中的冰冻切片组织病理学) 改善许多肿瘤手术的结果。但是，即使此过程生成的结果 比传统的福尔马林固定石蜡包埋(FFPE)组织病理学快得多，它仍然需要很长的时间- 敏感的手术，如头部和颈部肿瘤切除。在国际奥委会期间，手术经常需要暂停， 导致手术室时间和资源的昂贵浪费。因为国际奥委会通常需要快速 周转，一次只能准备和评估几个切片。外科医生之间的沟通不畅 而病理学家会导致抽样错误。冷冻伪影会导致组织学质量下降。这些 局限性增加了切片中遗漏重要组织病理学信息的风险，导致错误- 负边际，影响癌症手术的成功率和长期结果。因此，有一个 需要更快、更可靠的术中组织病理学评估。为了解决这些限制，我们 建议开发一种替代的组织学框架，用于术中组织病理学评估 结合了我们实验室开发的光学清理和3D成像技术。我们建议的产品将 提供术中标本的3D组织学信息，工作流程比 现有解决方案。该产品包括三个主要组成部分：1)快速组织处理流水线， 只需几次清洗即可使组织透明，2)用户友好的成像系统，可实现实时3D 透明整装临床标本的显微镜成像，3)软件用户界面，其 将3D数据转换为具有病理学家熟悉的颜色对比度的2D图像，并协助病理学家 解读数据。3D虚拟组织学已被提出作为基于切片的组织病理学的一种替代方案 提供更准确的诊断。然而，目前的3D虚拟组织病理学技术通常具有很高的 复杂性和周转时间长。我们的初步客户发现表明，这是一项极具挑战性的工作 对于组织学实验室来说，将这些新技术整合到现有的完善的工作流程中是因为 资本成本和复杂性。通过将样品制备简化为2-3次液体洗涤并提供插头- 只需最少的校准和维护即可使用3D成像设备，我们的产品将支持3D虚拟 比传统方法更容易、更快速、更准确的组织病理学。

Project Summary Intraoperative consultations (IOC, often refers to frozen-section histopathology during surgery) significantly improve the outcome of many oncological surgeries. However, even though this procedure generates results much faster than traditional formalin-fixed paraffin-embedded (FFPE) histopathology, it is still lengthy for time- sensitive surgeries such as head and neck tumor resections. Surgeries often need to be paused during the IOC, leading to an expensive waste of operating room time and resources. Because IOC usually requires a fast turnaround, only a few slices can be prepared and evaluated at a time. Miscommunication between surgeons and pathologists leads to sampling errors. Freezing artifacts result in degraded histology quality. These limitations increase the risk of missing important histopathology information in the slice, resulting in false- negative margins which affects the success rate and long-term outcomes of cancer surgeries. Thus, there is a need for faster and more reliable intraoperative histopathology evaluation. To address these limitations, we propose to develop an alternative histology framework for intraoperative histopathology assessment using a combination of optical clearing and 3D imaging technologies developed in our lab. Our proposed product will provide 3D histology information of intraoperative specimens with a faster and simpler workflow than the incumbent solution. The product comprises three major components: 1) a rapid tissue processing pipeline that makes tissue transparent with a few washes, 2) a user-friendly imaging system that enables real-time 3D microscopy imaging of the transparent whole-mount clinical specimens, 3) A software user interface that translates the 3D data into 2D images with pathologist-familiar color contrast and assists the pathologist in interpreting the data. 3D virtual histology has been proposed as an alternative to slice-based histopathology to provide more accurate diagnoses. However, current 3D virtual histopathology techniques often have high complexity and long turnaround time. Our preliminary customer discovery suggested it was highly challenging for histology labs to incorporate these new techniques into the existing well-established workflow due to high capital cost and complexity. By simplifying the sample preparation into 2-3 fluid washes and providing a plug- and-play 3D imaging device that requires minimal calibration and maintenance, our product will enable 3D virtual histopathology that is easier, faster, and more accurate than the traditional methods.