Acquisition of a Crystal Gryphon-LCP Crystallization Robot

摘要 针对NOT-GM-22-017《设备采购行政补充材料可用性通知》 对于NIGMS获奖者，我们要求资金购买水晶狮鹫-LCP结晶机器人。我们 ParentGrant旨在建立调节蛋白质合成关键步骤的结构基础：起始， 终止和核糖体再循环。而与抑制剂复合的核糖体的几种晶体结构 尽管已经有报道，但强调抑制核糖体翻译因子的机制仍然存在， 不太了解。使晶化的结构确定和速度加快，并降低成本 为了实现我们的目标，我们需要一个高通量的结晶系统， 条件所要求的系统提供足够高的吞吐量，不仅支持Gagnon实验室， 西利结构生物学中心（SCSB）内的其他X射线晶体学实验室， 样品材料，可靠地使用商业结晶筛选试剂盒（96孔SBS格式）或专门 创建筛选，使用96孔、24孔、坐滴或悬滴技术进行优化， 增加了微播种的能力，这对我们的项目至关重要。为了认识到 该仪器的UTMB内的结构生物学社区，SCSB正在致力于空间，长期 维护，以及仪器操作和用户培训人员。

ABSTRACT In response to NOT-GM-22-017, Notice of Availability of Administrative Supplements for Equipment Purchases for NIGMS Awardees, we request the funds to purchase the Crystal Gryphon-LCP Crystallization Robot. Our parent grant aims to establish the structural bases regulating the key steps of protein synthesis: initiation, termination, and ribosome recycling. While several crystal structures of the ribosome in complex with inhibitors have been reported, the mechanisms that underline inhibition of translation factors off the ribosome remain poorly understood. To enable structure determination and speed up as well as decrease the cost of crystallizing our targets, we need a high-throughput crystallization system that can efficiently identify successful crystallization conditions. The requested system offers sufficient high throughput to support not only the Gagnon lab, but also the other x-ray crystallography labs within the Sealy Center for Structural Biology (SCSB), requires minimal sample material, reliably uses either commercial crystallization screening kits (96-well SBS format) or specially created screens, performs optimization using 96-well, 24-well, sitting-drop, or hanging-drop techniques, and adds the capability of microseeding, which is proving essential to our project. In recognition of the broad benefit of this instrument to the structural biology community within UTMB, the SCSB is committing space, long-term maintenance, and staff for operation and user training of the instrument.