Micromanipulator systems, TransferMan 4r and FemtoJet 4i

摘要 在家长资助中，我们计划在Marko博士的实验室学习最先进的微操作系统，地址为 西北大学，然后在伊利诺伊大学厄巴纳-香槟分校建立了我们自己的一个。通过使用 在Marko实验室的微操作系统中，我们获得了许多关于减数分裂染色体的有趣数据 并在《通信生物学》上发表了一篇论文。此外，我们是第一个分离中期分裂相的小组 在小鼠卵母细胞中，I与染色体一起纺锤形。这将为研究染色体提供新的方法。 种族隔离和畸形。我们关于染色体间相互作用的发现也将建立一个新的范式 关于染色体分离。 我当地的合作研究员莱克班德博士在伊利诺伊大学有一个类似的微操作系统， 乌尔巴纳-香槟。然而，该系统缺乏自动微操作器。如果没有这两个操纵者，它 不可能捕获减数分裂染色体并进一步测量它们的生物物理性质。设置后 在本地升级系统，我的实验室可以培训更多的成员使用尖端技术，这是 对这些拟议项目的成功至关重要。 我们最近发现，对突变的精母细胞和卵母细胞的减数分裂染色体的测量可以 不能在马尔科博士的实验室进行，因为需要新鲜的突变睾丸和卵巢。变种人 精母细胞在从Urbana运往埃文斯顿后变得不健康。然而，所有的突变小鼠 线路目前在伊利诺伊大学厄巴纳-香槟分校维护。这意味着我们必须使用本地的 微操作系统测量突变精母细胞的染色体硬度和 卵母细胞。从这些突变体中收集的数据是拟议中的项目的基础。 综上所述，我们是第一个将微操作系统应用于减数分裂染色体的小组。因此， 当地系统将对完成拟议的项目至关重要，显著加快这些项目的速度，以及 促进有趣的发现。

Abstract In the parent grant, we plan to learn the state-of-the-art micromanipulation system in Dr. Marko's lab at Northwestern University and then set up our own one at the University of Illinois, Urbana-Champaign. By using the micromanipulation system in Marko's lab, we have obtained many interesting data on meiotic chromosomes and published one paper in Communications Biology. In addition, we are the first group who isolated metaphase I spindle with chromosomes in mouse oocytes. This will provide novel approaches to study chromosome segregation and abnormality. Our findings on inter-chromosomal interaction will also establish a new paradigm on chromosome segregation. My local co-investigator, Dr. Leckband, has a similar micromanipulation system at the University of Illinois, Urbana-Champaign. However, this system lacks automatic micromanipulators. Without the two manipulators, it is impossible to capture the meiotic chromosomes and further measure their biophysical properties. After setting up the system locally, more members from my lab can be trained to use the cutting-edge technology, which is essential for the success of these proposed projects. We recently found that the measurement of meiotic chromosomes from mutant spermatocytes and oocytes could not be conducted in Dr. Marko's lab because fresh mutant testes and ovaries are required. The mutant spermatocytes became unhealthy after the shipment from Urbana to Evanston. However, all the mutant mouse lines are currently maintained at the University of Illinois, Urbana-Champaign. This means we have to use a local micromanipulation system to measure the stiffness of the chromosomes from the mutant spermatocytes and oocytes. The data collected from these mutants is fundamental for the proposed projects. In summary, we are the first group to apply the micromanipulation system onto meiotic chromosomes. Thus, the local system will be vital for completion of the proposed projects, dramatically speed up these projects, and facilitate intriguing discoveries.