Cellular and Molecular Physiology Core

项目总结-细胞分子生理学核心 细胞和分子生理学核心是该中心的“工作马匹”，但它也继续充当 一个创新中心，为肝脏研究人员提供最新的实验模型。核心是 组织向肝脏中心调查人员提供技术专长、设备和人员，以便 为他们提供最先进的细胞和分子研究资源 具有成本效益的方式。Core在为肝脏研究开发新的细胞和动物模型方面有着悠久的历史。 目前，核心分为两个部分：(1)细胞分离和细胞培养部分和(2) 分子成分。这些细分为：(A)分离的细胞制剂，包括：肝细胞， 胆管细胞、内皮细胞、星状细胞、门静脉成纤维细胞和肝淋巴细胞，主要来自小鼠 还有老鼠。人体肝细胞也在可利用的情况下使用。超过3,400个分离的细胞 (B)短期和长期培养和细胞培养设施 (C)使用定量RT-PCR和红外成像检测的蛋白质和基因表达；(D)改变 肝相关细胞、细胞系和组织中基因表达的siRNA转染、腺病毒感染和 CRISPR技术；(E)提供各种肝病动物模型；(F)针对疾病的小鼠 以及来自IPSC、胆汁和初级肝组织的人肝有机类物质。通过集中这些程序 在核心设施中，成本和工作量大大降低，调查人员得到了高度严谨的保证， 重现性、质量控制和制剂往往可以同时使用一种以上 调查员。Mario Strazzabosco，医学博士，拥有超过25年的相关经验，指导这项工作 CORE与荣誉退休主任詹姆斯·L·博耶(James L.Boyer，MD)合作，他拥有40多年的 有使用这些准备和程序的经验。他们得到了罗米娜·菲奥罗托博士和 蔡时英博士，确保对其中许多项目的日常监督、可用性和调查人员建议 服务。

PROJECT SUMMARY – CELLULAR MOLECULAR PHYSIOLOGY CORE The Cellular and Molecular Physiology Core is the “work horse” of this Center, but it also continues to serve as an innovation hub to provide liver investigators with the most up-to-date experimental models. The core is organized to provide technical expertise, equipment and personnel to Liver Center Investigators in order to provide them with state of the art cell and molecular research resources in an efficient, quality-controlled and cost-effective manner. The Core has a long history of developing new cell and animal models for liver research. Currently, the Core is divided into two Components: (1) The Cell Isolation and Cell Culture Component and (2) The Molecular Component. These are subdivided into: (a) Isolated cell preparations, including: hepatocytes, cholangiocytes, endothelial cells, stellate cells, portal fibroblasts and hepatic lymphocytes, primarily from mice and rats. Human hepatocytes are also utilized when available. Over 3,400 separate cell isolations were performed during the current award period; (b) Cell culture facilities for short and long-term cultures and cell lines; (c) Protein and gene expression using Quantitative RT-PCR and Infrared imaging detection; (d) Altering gene expression in liver-related cells, cell lines and tissues using siRNA transfection, adenovirus infection, and CRISPR technologies; (e) providing a variety of animal models of liver disease and (f) disease-specific mouse and human liver organoids, derived from iPSC’s, bile, and primary liver tissue. By centralizing these procedures in a Core facility, cost and effort are dramatically reduced, investigators are assured of a high degree of rigor, reproducibility, and quality control, and preparations can often be used simultaneously by more than one investigator. Mario Strazzabosco, MD, PhD, who has more than 25 years of relevant experience, directs this core with the collaboration of the Emeritus Director, James L. Boyer, MD, who has more than 4 decades of experience working with these preparations and procedures. They are assisted by Romina Fiorotto, PhD, and Shi-Ying Cai, PhD, which assures daily supervision, availability, and advice to investigators for many of these services.