Physiology and Behavior Core

摘要-生理和行为（PB）核心 糖尿病和肥胖症的许多生理和行为原因、并发症和后果可以 在小鼠模型中进行评估。小鼠的关键生理和行为表型提供了有用的 结果的措施和翻译标记，以告知这些和其他新的治疗方法的发展 人体的代谢状况。生理学和行为（PB）核心的使命将是促进 了解行为表现、病理生理机制和系统后果 疾病。一系列独特、复杂和创新的体内试验和程序将与 实验结果和成果的规划、分析和解释咨询服务 在活的老鼠中测量。PB核心服务将补充国家MPMOD中的其他核心服务 财团和MMH和中心的动物核心。PB Core还将利用专业知识， 加州大学戴维斯分校的几个实验室、核心和服务中心的能力， 服务和资源，以支持研究人员使用中心的研究活小鼠。PB Core将 面向服务、面向客户和支持研究。PB核心将配备一名领导人， 组长、核心协调员、技术专家和5名科学顾问。 PB Core的使命将通过以下2个具体目标来完成： 具体目标1：提供创新、全面、高质量、最先进的生理和 肥胖和糖尿病表型的体内行为测试， 生物医学社区 具体目标2：为客户提供研究设计、试验选择、 和试验性规划，分析和解释结果和成果措施， 与糖尿病、肥胖和其他代谢紊乱有关的生理和行为。 根据这些具体目标，方案支助处核心将开展以下活动： ·提供全面的表型分析工具，用于眼、心血管、 肾脏和胃肠道功能在肥胖和糖尿病小鼠模型中的两种性别的小鼠; ·提供一系列测试，探索多个行为领域（一般健康，运动能力， 焦虑，情感和社会行为，以及记忆和学习），以了解肥胖如何影响小鼠的行为。 代谢紊乱影响神经生理和执行功能; ·通过以下方式对睡眠期间的运动、步态、睡眠阶段、纺锤波事件和呼吸暂停进行分析： 呼吸生理学与神经生理学配对，这将为 研究小鼠能量平衡的改变和MMH核心提供的补充服务; ·提供独特的创新服务，以评估受糖尿病影响的特殊生理功能 和/或肥胖症，包括小鼠的肠屏障功能、伤害感受、成像和神经病; ·提供最先进的技术，以观察和衡量项目的成果， 同时在体内进行行为和神经生理学研究; ·协助研究人员为出版物产生高质量和可重复的研究结果， 用于资助提案的试点数据，以及为人类临床试验提供信息的临床前发现; ·对新的和可定制的测试和程序的请求具有灵活性和响应能力; ·参与充满活力的计划，以帮助和培训新一代的科学家， 重点关注代表性不足的研究人员和生物医学研究的日益多样性。 在PB核心的所有活动中注入必要的控制，重复进行的测试，适当的 群体规模，使用男性和女性来解决性别作为生物变量的问题，以及其他方面与 严谨性和可重复性的原则。

ABSTRACT—PHYSIOLOGY AND BEHAVIOR (PB) CORE Numerous physiological and behavioral causes, complications and consequences of diabetes and obesity can be assessed in mouse models. Key physiological and behavioral phenotypes in mice are providing useful outcome measures and translational markers to inform the development of new treatments for these and other metabolic conditions in humans. The mission of the Physiology and Behavior (PB) Core will be to advance the understanding of behavioral manifestations and pathophysiological mechanisms and systemic consequences of disease. A selection of unique, complex, and innovative in vivo tests and procedures will be combined with consultative services on planning, analyzing, and interpretation of experimental results and outcomes measures in living mice. PB Core services will complement those of other Cores in the national MPMOD Consortium and the MMH and Animal Cores of the Center. The PB Core will also leverage the expertise and capabilities of several laboratories, cores, and service centers at UC Davis to provide ancillary analytical services and resources in support of investigators using the Center for studies on live mice. The PB Core will be service-oriented, client-facing, and research-supportive. The PB Core will be staffed by a Leader, co- Leader, Core Coordinators, Technical Specialists, and 5 Scientific Consultants. The mission of the PB Core will be accomplished through the following 2 Specific Aims: Specific Aim 1: Provide innovative, comprehensive, high quality, state-of-the art physiological and behavioral testing in vivo of obesity and diabetes phenotypes to investigators from across the biomedical community. Specific Aim 2: Provide clients with consultation services and advice on study design, test selection, and experimental planning, analysis and interpretation of results and outcomes measures, in the areas of physiology and behavior related to diabetes, obesity, and other metabolic disorders. Under these specific aims, the PB Core will carry out the following activities: • Providing comprehensive phenotyping tools for in vivo characterization of ocular, cardiovascular, kidney, and gastrointestinal functions in mouse models of obesity and diabetes in both sexes of mice; • Offering a battery of tests probing multiple behavioral domains (general health, motor capabilities, anxiety, affective and social behaviors, and memory and learning) in mice to understand how obesity and metabolic disorders influence neurophysiological and executive functions; • Conducting analyses of exercise, gait, sleeping stages, spindle events, and apneas during sleep by respiratory physiology paired with neurophysiology, which will provide essential information for studying alterations of energy balance in mice and complement services provided by the MMH Core; • Providing unique, innovative services to assess special physiological functions impacted by diabetes and/or obesity, including intestinal barrier function, nociception, imaging, and neuropathy in mice; • Offering state-of-the-art technologies to observe and measure outcomes in projects that pair behavioral with neurophysiology studies simultaneously in vivo; • Assisting investigators to generate high-quality and reproducible research results for publications, pilot data for grant proposals, and preclinical findings to inform human clinical trials; • Being flexible and responsive to requests for new and customizable tests and procedures; • Participating in the Vibrant Program to assist and train a new generation of scientists with a tailored focus toward underrepresented investigators and increasing diversity in biomedical research. Infused in all activities of the PB Core will be necessary controls, tests conducted in replicates, appropriate group sizes, use males and females to address sex as a biological variable, and other aspects consistent with the principles of rigor and reproducibility.