Basic Science Core - Imaging

德克萨斯生物医学In-TRAC研究成像核心摘要 研究成像核心将利用德克萨斯州Bied的关键优势，即NIH的强大队列- 为具有合作历史和不同成像平台经验的结核病调查人员提供资金。在- TRAC参与者将受益于获得西南国家灵长类研究中独特的NHP资源 中心(SNPRC)及其尖端成像能力。研究成像核心还将建立一个 向In-TRAC参与者介绍各种基于细胞和整体动物成像的教育课程 站台。目标1：提供共焦显微镜和光学方面的教学和实践培训计划 成像。In-TRAC参与者将学习多标记免疫荧光或荧光原位杂交 (FISH)共聚焦显微镜，允许对具有多个荧光色素的组织和细胞进行3-D或4-D评估 标签。目标2.提供尖端细胞免疫学技术的教学和实践培训 需要成像支持或计算分析。得克萨斯州生物医学、显微镜和分子科学 核心提供高维、多参数流式细胞术和分析以及BSL3分选 能力。资深结核病研究人员将提供活细胞成像(Cytation 5)和scRNAseq(10倍)培训 基因组学)。目标3：使用PET/CT和IVIS提供实时成像的教学和实践培训 系统。全身活体正电子发射断层扫描(PET)和X射线计算机断层扫描的培训 (CT)BSL3内啮齿动物的NHP成像和体内成像系统(IVIS)。TRAC内参与者将 参观UTHSCA的NHP MRI设施，了解该成像平台的优势和局限性。在… 研究成像核心的完成，参与者将能够有效地解释发布的数据， 使用NHP、啮齿动物、组织、细胞和RNA的成像方式；设计实验来测试它们自己的 假设；并参与使用适当动物模型的结核病研究。

TEXAS BIOMED IN-TRAC Research Imaging Core ABSTRACT The Research Imaging Core will leverage key strengths of Texas Biomed, namely a strong cohort of NIH- funded TB investigators with a history of collaboration and with experience in different imaging platforms. IN- TRAC participants will benefit from access to unique NHP resources in the Southwest National Primate Research Center (SNPRC) and its cutting edge imaging capabilities. The Research Imaging Core will also establish an education curriculum to introduce IN-TRAC participants to a variety of cell-based and whole animal imaging platforms. AIM 1: Provide didactic and hands-on training program in confocal microscopy and optical imaging. IN-TRAC participants will learn multi-label immunofluorescence or fluorescence in-situ hybridization (FISH) confocal microscopy, which allows for 3- or 4-D evaluation of tissues and cells with multiple fluorochrome labels. AIM 2. Provide didactic and hands-on training in cutting-edge cellular immunology techniques requiring imaging support or computational analysis. The Texas Biomed Biology, Microscopy and Molecular Cores provide high-dimensional, multi-parameter flow cytometry and analysis as well as BSL3 sorting capabilities. Established TB researchers will provide training live cell imaging (Cytation 5) and scRNAseq (10x Genomics) in BSL3. AIM 3: Provide didactic and hands-on training in live imaging using PET/CT and IVIS systems. Training in whole-body live Positron Emission Tomography (PET) and X-ray Computed Tomography (CT) imaging of NHP and in-vivo imaging system (IVIS) of rodents within the BSL3. IN-TRAC participants will extern at the NHP MRI facilities at UTHSCA to learn the advantages and limitations of this imaging platform. At the completion of the Research Imaging Core, participants will be able to effectively interpret published data that use imaging modalities for NHP, rodents, tissues, cells and RNA; design experiments to test their own hypothesis; and participate in TB research that uses the appropriate animal model.