Basic Science Core - Imaging

基础科学核心 - 成像

• 批准号: 10431470
• 负责人: Deepak Kaushal
• 金额: $ 19.99万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-10 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10431470
• 关键词: Address; Animal Model; Animals; Basic Science; Biology; Biomedical Research; Caring; Cell Separation; Cells; Cellular Immunology; Cities; Clinical; Clinical Management; Clinical Services; Collaborations; Communities; Complement; Computational algorithm; Computer Analysis; Confocal Microscopy; Containment; Data; Disadvantaged; Discipline; Education; Educational Curriculum; Emission-Computed Tomography; Equipment; Evaluation; Flow Cytometry; Fluorescent in Situ Hybridization; Fluorochrome; Freezing; Funding; Funding Agency; Genomics; Goals; Health Professional; Image; Image Analysis; Immunofluorescence Immunologic; Inpatients; Institutes; Knowledge; Label; Laboratories; Lasers; Leadership; Learning; Light; Magnetic Resonance Imaging; Mexico; Microscopy; Molecular; Optics; Outpatients; Paraffin; Participant; Positron-Emission Tomography; Primates; Publishing; RNA; Recording of previous events; Research; Research Institute; Research Personnel; Resources; Robotics; Rodent; Science; Scientist; Services; Sorting - Cell Movement; Structure; System; Techniques; Testing; Texas; Thick; Tissues; Training; Training Programs; Tuberculosis; United States National Institutes of Health; Work; X-Ray Computed Tomography; animal safety; base; biocontainment facility; cohort; design; experience; experimental study; high dimensionality; imaging capabilities; imaging modality; imaging platform; imaging study; in vivo imaging system; live cell imaging; microscopic imaging; multidisciplinary; next generation; optical imaging; peer; prevent; programs; prospective; skills; tool; whole animal imaging

TEXAS BIOMED IN-TRAC Research Imaging Core ABSTRACT The Research Imaging Core will leverage key strengths of Texas Biomed, namely a strong cohort of NIH- funded TB investigators with a history of collaboration and with experience in different imaging platforms. IN- TRAC participants will benefit from access to unique NHP resources in the Southwest National Primate Research Center (SNPRC) and its cutting edge imaging capabilities. The Research Imaging Core will also establish an education curriculum to introduce IN-TRAC participants to a variety of cell-based and whole animal imaging platforms. AIM 1: Provide didactic and hands-on training program in confocal microscopy and optical imaging. IN-TRAC participants will learn multi-label immunofluorescence or fluorescence in-situ hybridization (FISH) confocal microscopy, which allows for 3- or 4-D evaluation of tissues and cells with multiple fluorochrome labels. AIM 2. Provide didactic and hands-on training in cutting-edge cellular immunology techniques requiring imaging support or computational analysis. The Texas Biomed Biology, Microscopy and Molecular Cores provide high-dimensional, multi-parameter flow cytometry and analysis as well as BSL3 sorting capabilities. Established TB researchers will provide training live cell imaging (Cytation 5) and scRNAseq (10x Genomics) in BSL3. AIM 3: Provide didactic and hands-on training in live imaging using PET/CT and IVIS systems. Training in whole-body live Positron Emission Tomography (PET) and X-ray Computed Tomography (CT) imaging of NHP and in-vivo imaging system (IVIS) of rodents within the BSL3. IN-TRAC participants will extern at the NHP MRI facilities at UTHSCA to learn the advantages and limitations of this imaging platform. At the completion of the Research Imaging Core, participants will be able to effectively interpret published data that use imaging modalities for NHP, rodents, tissues, cells and RNA; design experiments to test their own hypothesis; and participate in TB research that uses the appropriate animal model.

德克萨斯州BIOMED IN-TRAC研究成像核心摘要 研究成像核心将利用德克萨斯生物医学的关键优势，即NIH的强大队列， 资助的结核病研究人员，具有合作历史和不同成像平台的经验。IN- TRAC参与者将受益于获得西南国家灵长类动物研究的独特NHP资源 中心（SNPRC）及其尖端的成像能力。研究成像核心还将建立一个 教育课程，向IN-TRAC参与者介绍各种基于细胞和整体动物成像 平台目的1：提供共聚焦显微镜和光学显微镜的教学和实践培训计划 显像IN-TRAC参与者将学习多标记免疫荧光或荧光原位杂交 （FISH）共聚焦显微镜，允许使用多种荧光染料对组织和细胞进行3-D或4-D评估 标签AIM 2.提供尖端细胞免疫学技术的教学和实践培训 需要成像支持或计算分析。德克萨斯生物医学生物学、显微镜和分子生物学 核心提供高维、多参数流式细胞术和分析以及BSL 3分选 能力的成熟的结核病研究人员将提供培训活细胞成像（Cytation 5）和scRNAseq（10倍） 基因组学）在BSL 3中。目标3：提供使用PET/CT和IVIS进行实时成像的教学和实践培训 系统.全身活体正电子发射断层扫描（PET）和X射线计算机断层扫描培训 (CT)BSL 3内啮齿动物的NHP和体内成像系统（IVIS）成像。核心预算资源调拨目标参与者将 在UTHSCA的NHP MRI设施外，了解该成像平台的优点和局限性。在 研究成像核心的完成，参与者将能够有效地解释已发表的数据， 使用NHP，啮齿动物，组织，细胞和RNA的成像模式;设计实验来测试他们自己的 假设;并参与使用适当动物模型的结核病研究。