Cell Biology Core

项目总结-细胞生物学核心 细胞生物学自1977年成立以来一直是DRTC的组成部分。它采取了多种形式， 在过去的40年里，最近专注于胰岛和β细胞的研究。但近年来 并且，在本申请中继续，细胞生物学核心已经扩展到还包括以下研究： 脂肪细胞、肝细胞以及来自糖尿病和非糖尿病人的先天性和适应性免疫细胞， 小鼠核心提供了广泛的服务，在细胞为基础的系统，阐明胰岛内分泌的作用， 细胞，脂肪细胞，肝细胞和免疫细胞在健康和疾病的研究重点是使用初级 人类和小鼠的细胞。此外，通过此次更新，Core将在斑马鱼中提供精密的模型 在体内和真实的时间研究致糖尿病环境（胰岛素抵抗、胰岛素缺乏）的影响 代谢活性细胞，包括胰岛、肝细胞、肾细胞和先天免疫细胞。核心 提供有关研究设计、方法和数据解释的建议。因为很多技术都是新的 调查人员，核心还提供直接的实践支持和培训，以确保成功和及时 完成实验。该核心以芝加哥大学和伊利诺伊大学为中心， 芝加哥。这两个小组定期举行会议，并制定了标准作业程序，以确保 用户可以获得高质量的学习支持。核心还不断致力于培训， 对调查人员、研究员、学生和研究技术人员进行核心程序方面的培训。细胞生物学 核心是DRTC的组成部分，因此通过促进基于糖尿病的 对小鼠和人类原代细胞和组织的研究。此外，它还允许在 遗传学和基因组学核心将在生物学背景下进行测试。核心的目标是： 1.为了提供胰岛、脂肪细胞、肝细胞以及先天性和适应性免疫细胞（例如， 巨噬细胞、嗜中性粒细胞、T细胞），以及胰岛素瘤， 前脂肪细胞和iPSC（来自非糖尿病患者和单基因型糖尿病患者）细胞系。 2.不断改进新的实验方法（例如使用iPSC衍生的细胞）， 为发现提供更好的生理和病理生理背景。 3.积极促进DRTC成员之间的互动和合作，吸引外部 糖尿病研究领域的研究人员通过突出糖尿病相关实验的优势， 交叉主题和问题的研究系统。

PROJECT SUMMARY – CELL BIOLOGY CORE Cell Biology has been a component of the DRTC since its inception in 1977. It has taken many forms over the past 40 years, most recently focusing on studies of pancreatic islets and beta cells. However, in recent years and, continuing in this application, the Cell Biology Core has been expanded to also include studies of adipocytes, hepatocytes, and innate and adaptive immune cells from diabetic and non-diabetic humans and mice. The Core provides a broad range of services in cell-based systems to elucidate the roles of islet endocrine cells, adipocytes, hepatocytes and immune cells in health and disease with an emphasis of studies using primary cells from humans and mice. In addition, with this renewal, the Core will provide sophisticated models in zebrafish to study in vivo and in real time the effect of the diabetogenic environment (insulin resistance, insulin deficiency) on metabolically active cells, including islets, hepatocytes, kidney cells, and innate immune cells. The Core provides advice on study design, methods, and data interpretation. Because many of the techniques are new to investigators, the Core also provides direct hands-on support and training to ensure the successful and timely completion of experiments. This Core is centered at the University of Chicago and the University of Illinois at Chicago. The two groups meet regularly and have developed standard operating procedures to ensure that Users receive quality support for their studies. The Core also has an ongoing commitment to training and has trained investigators, fellows, students and research technicians in the procedures of the Core. The Cell Biology Core is an integral part of the DRTC, and thus synergizes with the Physiology Core by facilitating diabetes-based research on primary cells and tissues from mice and humans. Moreover, it allows discoveries made in the Genetics and Genomics Core to be tested in a biological context. The Aims of the Core are: 1. To provide pancreatic islets, adipocytes, hepatocytes, and innate and adaptive immune cells (e.g. macrophages, neutrophils, T-cells) from normal and diabetic humans, mice, and zebrafish, as well as insulinoma, pre-adipocyte, and iPSC (from non-diabetic patients and patients with monogenic forms of diabetes) cell lines. 2. To continuously improve new experimental approaches (e.g. use of cells derived from iPSCs) that can provide better physiological and pathophysiological context for discovery. 3. To actively promote interaction and collaboration among DRTC members and attract outside investigators to the field of diabetes research by highlighting the advantages of diabetes-relevant experimental systems for studies of cross-cutting themes and questions.