Core A: Mouse Core

STAT3变异体作为免疫耐受小鼠核心的变阻器 项目摘要 使用转基因小鼠彻底改变了复杂生物过程的研究，包括 免疫耐受和自身免疫。本P01中的所有项目都严重依赖于使用转基因技术。 转基因、基因敲除和自身免疫倾向小鼠品系。许多自身免疫倾向和免疫缺陷 生产线需要特定的专业知识和最佳研究条件，包括无病原体的住房 环境.此外，当处理这些动物时，这些动物的繁殖和护理可能变得复杂。 多种基因修饰品系和不同基因品系的小鼠。一个鼠标核心是设想， 帮助简化和提高效率与遗传复杂的线的产生和维护， 一个健康无病原体的环境由于几个P01项目依赖于NOD小鼠， 糖尿病模型和新型STAT3功能获得（GOF）小鼠系，许多核心工作将 围绕这些线路的维护、分布和使用，结合其他相关免疫 系统报告基因或免疫缺陷系。这样一来，核心就可以帮助确保质量，遗传血统， 以及共享P01研究者合作研究所需的动物。

STAT3 Variants as a Rheostat of Immune Tolerance Mouse Core Project Summary The use of genetically altered mice has revolutionized the study of complex biological processes, including immune tolerance and autoimmunity. All the projects in this P01 rely heavily on the use of genetically modified transgenic, knockout, and autoimmune-prone mouse strains. Many autoimmune prone and immunodeficient lines require specific expertise and conditions for optimal research, including housing in pathogen-free environments. Additionally, breeding and care of such animals can become complex when dealing with multiple genetically modified lines and different genetic strains of mice. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. Because of the reliance of several P01 projects on the NOD mouse model of diabetes and the novel STAT3 gain of function (GOF) mouse lines, much of the Core efforts will revolve around the maintenance, distribution and use of these lines in combination with other relevant immune system reporters or immunodeficient lines. In this way, the Core can help ensure the quality, genetic lineage, and sharing of animals needed for the collaborative studies of the P01 investigators.