Genomic Sciences Core

基因组科学核心(GSC)的目标是为老龄化和老年科学的研究人员提供 能够获得针对生物学的最先进的基因组、表观基因组和转录组分析的国家 老龄化研究问题，通常不是由机构核心提供的。具体地说，GSC将 提供线粒体基因组分析、DNA修饰(甲基化/羟甲基化)、 和单细胞转录组。在与老年信息核心的协调下，这些服务将采取 调查员提交的样本从数据生成到分析和解释。在前一个周期中，我们的 表观基因组和线粒体基因组服务得到了广泛使用，并将继续提供。新服务 将整合全基因组表观基因组分析，区分甲基化和羟甲基化，以及 一套单细胞RNA-Seq服务。将服务集中在这些领域的理由是：1)积累 线粒体DNA突变/缺失和拷贝数变化是一种中心机制假说 随着年龄的增长线粒体功能障碍。2)表观遗传机制是一种保守的、潜在的致病机制， 老化的因素。越来越多的证据表明，表观基因组不仅会随着年龄的增长而变化，而且会随着年龄的增长而变化 衰老干预可以防止与年龄相关的表观基因组变化。3)现代分子生物学已经开启 了解细胞异质性的新途径--当前老年科学研究的核心领域 (例如，细胞衰老)。单细胞转录方法超越了整个组织的大宗RNA序列， 单个细胞类型和子类型。GSC服务设计的核心是要采用的生物信息学工作流程 调查人员从抽样到充分分析数据。GSC将有三个目标：1)执行精确的量化 利用先进的表观遗传学进行从基因组范围到基因特异性的DNA修饰分析 技术，2)测量线粒体基因组异质性和综合变异的拷贝数 分析和绝对定量，以及3)用于细胞异质性分析的单细胞转录组学。GSC 服务的设计使老龄化研究人员可以利用特定的分析或整体 满足他们的实验需求的工作流，并且几乎所有服务都可以在存储的核上执行 酸/细胞。基因组科学核心开发了一套与衰老研究高度相关的工具，这些工具 不能广泛提供给调查人员，无论是在他们自己的实验室里，还是通过机构核心设施，都是在 在仪器和专业知识方面，满足了对外地资源提供者的需求。此外， 与老年信息核心的互动提供了一个工作流程，在这个工作流程中，老年科学研究人员不仅可以从 全国各地都有由GSC生成的数据，但这些数据被分析为调查员可用的形式。

The goal of the Genomic Sciences Core (GSC) is to provide researchers in aging and geroscience across the country with access to state-of-the-art genomic, epigenomic, and transcriptomic analyses targeted to biology of aging research questions and that are not typically provided by institutional cores. Specifically, the GSC will offer services in the analysis of mitochondrial genomes, DNA modifications (methylation/hydroxymethylation), and single-cell transcriptomics. In coordination with the GeroInformatics Core, these services will take investigator-submitted samples from data generation to analysis and interpretation. In the previous cycle, our epigenomic and mitochondrial genomic services were widely used and will continue to be offered. New services will integrate whole genome epigenomic analyses, differentiation of methylation from hydroxymethylation and a suite of single cell RNA-Seq services. The rationale for focusing services in these areas are: 1) Accumulation of mitochondrial DNA mutations/deletions and copy number changes are a central mechanistic hypothesis in mitochondrial dysfunction with aging. 2) Epigenetic mechanisms are a conserved, and potentially causative, factor in aging. Growing evidence demonstrates that not only does the epigenome change with aging, but anti- aging interventions can prevent age-related changes to the epigenome. 3) Modern molecular biology has opened a new door to understand cellular heterogeneity a research area at the heart of current geroscience research (e.g., cellular senescence). Single-cell transcriptomic approaches move past bulk RNA-Seq of whole tissues to individual cell types and sub-types. Central to the design of the GSC services are bioinformatic workflows to take investigators from sample to fully analyzed data. The GSC will have three aims: 1) Perform quantitatively precise DNA modification analyses, ranging from genome-wide to gene-specific using advanced epigenetic technologies, 2) Measure mitochondrial genome heteroplasmy and copy number for comprehensive variant analysis and absolute quantitation, and 3) Single-cell transcriptomics for analysis of cellular heterogeneity. GSC services are designed such that aging research investigators can take advantage of specific analyses or whole workflows as meets their experimental needs, and almost all services can be performed on stored nucleic acids/cells. The Genomic Sciences Core has developed a set of tools highly relevant to aging research that are not widely available to investigators, either in their own laboratories or through institutional core facilities, both in terms of instrumentation and expertise, meeting a need for a resource provider in the field. Furthermore, the interaction with the GeroInformatics Core provides a workflow where not only can geroscience investigators from across the country have data generated by the GSC but it is analyzed into an investigator-usable form.