Microbiology, Immunology, Animal Modeling and Imaging

摘要- JHU TRAC微生物学、免疫学、动物建模和成像（迈阿密）核心 约翰霍普金斯大学（JHU）TRAC微生物学、免疫学、动物建模和成像 （迈阿密）核心，主要由约翰霍普金斯结核病中心的实验室组成 研究（CTR）和中心感染和炎症成像研究（Ci 3R），将提供广泛的 支持JHU TRAC使命的实验室工具小组。由联合主任博士领导。 William Bishai和Sanjay Jain，并由另外10名高度成就的核心教师协助 结核病（TB）和非结核病研究者，迈阿密核心将提供数十年的综合研究 和指导专业知识，以及获得尖端实验室技术，以实现培训 （a）微生物学、分子遗传学和生物安全三级 （BSL 3）结核分枝杆菌的处理;（B）评估宿主对TB反应的免疫学工具 感染和疾病;以及（c）多种临床相关的TB动物模型（小鼠、豚鼠和 兔），以及用CT、PET和SPECT进行体内成像的能力。 迈阿密核心将协助新研究者（NI）和早期研究者（ESI）进行规划， 执行和解释所有临床前研究的数据，包括结核病的小动物模型、小鼠 育种，先进的全身活体体内成像方法，代谢组学和单细胞RNA （scRNA）测序。除了基于实验室的M。结核 微生物学、免疫学和发病机制，迈阿密核心将通过提供新的 主题（例如，结核病-艾滋病病毒合并感染、环境暴露和结核病易感性、结核病的性别差异 敏感性和先进的动物成像），目前无法通过CTR获得， JHU TRAC的独特之处此外，我们将利用众多独立和机构赠款， 进一步加强JHU TRAC内的基础科学能力。 迈阿密核心工作人员将为所有动物提供科学和技术领导以及运营监督， 研究计划的相关研究。迈阿密核心教师也将参加每周的TRAC 研讨会系列，提供结核病发病机制和新型成像工具的新研究的科学重点， 以及TRAC赞助的NI/ESI、TRAC K俱乐部和K2 R俱乐部的写作责任小组， 以及年度结核病科学会议和TRAC数据博览会。一个集中的核心对促进协同作用至关重要 与其他JHU TRAC核心，并提供集成，效率和易用性，将最大限度地 对参与JHU TRAC的所有初级和已确立研究者的价值和效用。总的来说，迈阿密 核心将通过整合专业知识、基础设施和功能来实现 提供世界一流的微生物学，免疫学，动物模型和成像支持， JHU TRAC，以及为学员，初级教师和结核病新研究人员提供研究机会。

ABSTRACT – JHU TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core The Johns Hopkins University (JHU) TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core, comprising laboratories primarily within the Johns Hopkins Center for Tuberculosis Research (CTR) and the Center for Infection and Inflammation Imaging Research (Ci3R), will offer a broad panel of laboratory-based tools to support the mission of the JHU TRAC. Headed by Co-Directors Drs. William Bishai and Sanjay Jain and assisted by a Core faculty of 10 additional highly accomplished tuberculosis (TB) and non-TB investigators, the MIAMI Core will provide decades of combined research and mentoring expertise, as well as access to cutting-edge laboratory technologies to enable training opportunities in and support for TB research in: (a) Microbiology, molecular genetics and biosafety level-3 (BSL3) handling of Mycobacterium tuberculosis; (b) Immunologic tools to assess host responses to TB infection and diseases; and (c) Multiple clinically-relevant animal models of TB (mouse, guinea pig, and rabbit), alongside the capacity to conduct in vivo imaging with CT, PET, and SPECT. The MIAMI Core will assist New Investigators (NI) and Early Stage Investigators (ESI) in planning, executing and interpreting the data from all preclinical studies, including small animal models of TB, mouse breeding, advanced whole-body live in vivo imaging approaches, metabolomics, and single-cell RNA (scRNA) sequencing. In addition to standard aspects of laboratory-based research on M. tuberculosis microbiology, immunology and pathogenesis, the MIAMI core will provide added value by offering novel topics (e.g., TB-HIV co-infection, environmental exposures and TB susceptibility, sex differences in TB susceptibility, and advanced animal imaging), which are not currently available through the CTR and are unique to the JHU TRAC. Furthermore, we will leverage numerous independent and institutional grants to further strengthen basic science capabilities within the JHU TRAC. The MIAMI Core staff will provide scientific and technical leadership and operational oversight to all animal- related studies of the research program. MIAMI Core faculty will also participate in the weekly TRAC Seminar Series, providing a scientific focus on novel research in TB pathogenesis and novel imaging tools, as well as the TRAC-sponsored Writing Accountability Groups for NI/ESI, TRAC K-Club and K2R Club, and annual TB Scientific Meeting and TRAC Data Fair. A centralized Core is essential to promote synergy with the other JHU TRAC Cores and to provide integration, efficiency, and ease of use that will maximize value and utility for all junior and established Investigators involved in the JHU TRAC. Overall, the MIAMI Core will add significant value through integration of expertise, infrastructure, and capabilities to achieve the goal of providing world-class microbiology, immunology, animal models, and imaging support to the JHU TRAC, as well as research opportunities to trainees, junior faculty, and researchers new to TB.