Radiation and Free Radical

辐射自由基研究核心(RFRRC)促进霍尔登综合癌症中心 (HCCC)需要辐射服务或定量分析方法的研究人员调查 以及操纵氧化还原状态和基于氧化还原的生物变化。它既为HCCC成员服务，也为 其他机构的癌症研究人员。RFRRC自1947年以来一直在运作，并由 作者：Douglas Spitz博士；Frederick Domann，Jr博士；Garry Buettner博士；Prabhat Goswami， 他们都在关注自由基癌症方面有丰富的经验和国际声誉。 研究。RFRRC提供三项基本服务。电离辐射服务由以下人员补充 磷成像和细胞周期分析工具对于了解基本的细胞行为和 对放射和化疗的反应。电子顺磁共振波谱及其他 分析化学检测方法可用于测量自由基、单线态氧、 小分子抗氧化剂、一氧化氮和一系列相关氧化剂与氧化损伤 产品。抗氧化剂酶服务使人们可以轻松地获得修饰和 测量负责促氧化剂形成和氧化损伤的分子。主要装备 RFRRC中可用的包括几个放射源、一个海马分析仪、各种类型的 分光光度计、缺氧室和用于高效液相分离和分析物检测的设备。 RFRRC为HCCC成员提供了方便地获取专业知识、试剂、 在高度协作和有益的环境中提供设备和资源。

The Radiation Free Radical Research Core (RFRRC) facilitates Holden Comprehensive Cancer Center (HCCC) researcher investigations that require radiation services or quantitative methods for analyzing and manipulating redox status and redox based biological changes. It serves both HCCC members and cancer researchers at other institutions. The RFRRC has been in operation since 1947, and is directed by Douglas Spitz, PhD; Frederick Domann, Jr, PhD; Garry Buettner, PhD; and Prabhat Goswami, PhD all of whom have extensive experience and international reputations in free radical-focused cancer research. The RFRRC provides three basic services. Ionizing radiation services are supplemented by phosphorimaging and cell-cycle analytical tools critical to understanding basic cellular behavior and responses to radiation and chemotherapy. Electron paramagnetic resonance spectroscopy and other analytical chemistry detection methodologies are available for measuring free radicals, singlet oxygen, small molecule antioxidants, nitric oxide and the array of related oxidants and oxidative damage products. Antioxidant enzyme services provide easy access to technologies for modifying and measuring molecules responsible for pro-oxidant formation and oxidative damage. Major equipment available in the RFRRC includes several radiation sources, a Seahorse analyzer, various types of spectrophotometers, hypoxia chambers, and equipment for HPLC separation and analyte detection. The RFRRC provides HCCC members with easy access to specialized knowledge, reagents, equipment and resources in a highly collaborative and helpful environment.