Cannabidiol and Macrophage Chronic Inflammation in a Virally Suppressed Rhesus Macaque Model

项目总结/摘要 该项目旨在了解大麻二酚对肠道细菌组成变化的影响， 抑制SIV恒河猴模型，以支持Rosado-Franco博士的培训，并最终过渡为 一位独立的药物滥用艾滋病研究员大麻二酚是最丰富的非精神药物 大麻植物中的大麻素及其在生活中的人中作为预防性抗炎治疗的研究 艾滋病是一个很大的问题。在本多元化补充提案中，我们有兴趣了解， 肠道细菌丰度的变化与髓样细胞活化或抗病毒药物的调节有关。 签名.来自健康或感染SIV并每日接受cART治疗的恒河猴的粪便和/或 CBD将被纵向收集。将提取基因组DNA，并将16 S和IT-S2基因 使用Illumina测序进行测序。分类概况和α和β多样性的差异将是 在不同的群体之间决定。这将有助于我们了解合适的细菌组成， 更健康的肠道和大脑免疫力在HIV猴疾病模型。在导师的指导下， 威廉姆斯和西尔斯，他的指导团队，以及拟议的培训和专业发展活动， 博士Rosado-Franco将在2023年9月向NIDA提交K 01申请，以促进 向独立的过渡。

PROJECT SUMMARY/ABSTRACT This project aims to understand the impact of cannabidiol on changes in gut bacterial compositions in a virally suppressed SIV rhesus macaque model to support the training of Dr. Rosado-Franco and ultimate transition as an independent substance abuse HIV researcher. Cannabidiol is the most abundant non-psychotropic cannabinoid from the Cannabis plant and its studies as adjunctive anti-inflammatory therapy in people living with HIV is of major interest. In this Diversity Supplement Proposal, we are interested in understanding if changes in the gut bacterial abundance are linked to myeloid cell activation or by modulation of antiviral signatures. Feces from Rhesus Macaque either healthy or infected with SIV and treated daily with cART and/or CBD will be collected longitudinally. Genomic DNA will be extracted, and 16S and IT-S 2 genes will be sequenced using Illumina sequencing. Differences in taxonomic profiles and alpha and beta diversity will be determined between different groups. This will help us understand the appropriate bacterial composition for healthier gut and brain immunity in a HIV monkey disease model. With the guidance of his mentors Drs. Williams and Sears, his mentoring team, and the proposed training and professional development activities, Dr. Rosado-Franco will be well prepared to submit a K01 application to NIDA in September 2023 to facilitate his transition to independence.