Selenium metabolism in cold-induced adaptive thermogenesis

摘要 本行政补充中提出的研究，以家长赠款DK 128390旨在促进科学的 Sydonie Swanson的研究和职业发展，他是一名具有西班牙裔血统的学士后候选人 寻求获得研究经验。家长补助金的重点是确定硒回收的作用， 调节棕色脂肪组织中的硒蛋白酶GPX 1和DIO 2， 有助于体内冷诱导的适应性产热，使用全身和棕色脂肪细胞特异性 硒再循环酶硒代半胱氨酸裂解酶（SCLY）的敲除小鼠模型。这一行政 补充将通过追求这些研究目标来补充这些研究：1）确定 硒和SCLY在棕色脂肪细胞分化中的作用; 2）确定前脂肪细胞的处理是否 与硒或SCLY的活性控制硒蛋白GPX 4的表达和活性，影响 对铁凋亡的反应， 在分化的棕色脂肪细胞中，铁依赖性细胞死亡机制。的 悉尼的研究和职业发展计划侧重于技术培训（例如，组织学，显微镜， 基因编辑，生物信息学）和专业（例如，实验设计、科学写作和口头 演讲）技能在协作环境中，将提供一个强大的研究经验，并有利于她 成功融入医疗队伍。

ABSTRACT The studies proposed in this Administrative Supplement to parent grant DK128390 aim to promote the scientific research and career development of Sydonie Swanson, a Postbaccalaureate candidate with a Hispanic heritage seeking to gain research experience. The parent grant focuses on determining the role of selenium recycling in regulating selenoprotein enzymes GPX1 and DIO2 in brown adipose tissue and how the recycling mechanism contributes to cold-induced adaptive thermogenesis in vivo, using whole-body and brown adipocyte-specific knockout mouse models of the selenium recycling enzyme selenocysteine lyase (SCLY). This Administrative Supplement will complement these studies by pursuing these Research Aims: 1) To determine the role of selenium and SCLY in the differentiation of brown adipocytes; and 2) To determine if treatment of pre-adipocytes with selenium or the activity of SCLY controls the expression and activity of selenoprotein GPX4, impacting responses to ferroptosis, an iron-dependent cell death mechanism, in differentiated brown adipocytes. The Research and Career Development Plan for Sydonie focuses on training technical (e.g., histology, microscopy, gene editing, bioinformatics) and professional (e.g., experimental design, scientific writing, and oral presentations) skills in a collaborative environment that will provide a robust research experience and favor her successful inclusion in the medical workforce.