Animal and Bioinformatics Core

项目摘要 动物核心（核心C）将通过生成所有小鼠来提供集中服务 中心项目所需。核心集中供应老鼠将确保 使用具有相同遗传背景的小鼠以最小化遗传背景的潜在变化。 表型、基因组或表观基因组分析。核心人员都很好， 接受过HFD治疗的培训，擅长监测发育和健康状况， GTT和ITT的测量将确保一致的数据质量和再现性。 具体地，核心C将产生高脂肪饮食（HFD）和正常饮食（ND）喂养的野生型小鼠。 和转基因小鼠，收集表型数据，并准备精子和组织样本， 两个基本项目。项目2和3都将研究HFD和/或不活动的影响 精子表观基因组和后代健康。WT或转基因（TU示踪剂和 KitcopGFP）小鼠将接受HFD或ND治疗3个月，项目3还需要 通过用ND喂养HFD处理的小鼠并引入 转轮分析精子表观基因组改变对HFD的影响 和/或运动时，治疗的雄性小鼠需要与WT雌性小鼠交配以产生 F1代。核心C将维持小鼠集落，进行所有处理，收集 表型数据，并提供实验所需的精子和组织样本 在项目2和3中提出。

Project Summary The Animal Core (Core C) will provide centralized services by generating all the mice needed for the Center projects. A centralized supply of mice by the Core will ensure that mice with the same genetic background are used to minimize potential variations in phenotypic, genomic or epigenomic analyses. The fact that Core personnel are well trained for HFD treatment, skillful in monitoring development and health status, and measurement of GTT and ITT will ensure consistent data quality and reproducibility. Specifically, Core C will generate high fat diet (HFD)- and normal diet (ND)-fed wild-type and transgenic mice, collect phenotypic data and prepare sperm and tissue samples for the two basic projects. Both projects 2 and 3 will work on effects of HFD and/or inactivity on sperm epigenome and offspring health. Both WT or transgenic (TU tracer and KitcopGFP) mice will be on a HFD or a ND for 3 months, and Project 3 also needs to reverse the HFD effect by feeding HFD-treated mice with ND and introducing to a running wheel. To analyze the effects of altered sperm epigenome in response to HFD and/or exercise, the treated male mice need to be bred with WT female mice to generate F1 progeny. Core C will maintain the mouse colonies, perform all the treatments, collect phenotypic data and provide sperm and tissue samples needed for the experiments proposed in Projects 2 and 3.