Core F: Systems Biology & Biostatistics

项目概要/摘要-系统生物学和生物统计学核心F RUSTBELT CFAR系统生物学和生物统计学核心F为广泛的CFAR提供支持 研究人员通过提供新的多组学技术，先进的生物统计学和计算分析“大”， 数据，”功能免疫学研究的更坚实基础，以及全面的“组学数据管理 的训练平台。核心F的关键使命是提供系统生物学方法， 整合宿主免疫反应的不同层次，从免疫反应的分子触发物到 转录签名和与常驻微生物群的相互作用。我们将继续支持功能 免疫学研究仍然是HIV研究的基础。核心帮助研究人员进行研究严谨性 通过实施研究设计、分析和公众分享的最佳实践， 结果在目标1中，核心F将支持艾滋病毒免疫学和发病机制中的基因组学和微生物组学研究 通过为用户提供一个强大的技术平台，通过RNA-seq进行转录分析， HIV感染的临床和动物模型的测序。在目标2中，核心F提供培训和咨询， 功能免疫学以及集中访问最先进的免疫技术，协议， 从血液和组织中分离、操作、培养和扩增原代免疫细胞的培训， 从而支持RUSTBELTCFAR的艾滋病毒传播、性别研究、治疗和发病机制 翻译目标。在目标3中，核心F通过提供集中的 使用高容量测定法， 分析工作流程以及公共可用的“omic”数据，以建立出版物和数据库。在Aim 4中 这项服务职能包括收集和编制用于协调的经济和功能数据， 分发给研究者，收集分析和研究所需的相关去识别患者注释 出版物Core F进行生物统计相关分析，以确定保护性免疫的关键驱动因素 回应，协助设计验证策略和撰写手稿，并提供元数据和数据 将结果上传到公共数据库的标准化管道。通过提供基本的免疫建模 支持与多组学方法配对，以识别宿主-病毒界面的关键驱动因素或生物标志物， 核心F将促进必要的完整系统生物学方法，以支持CFAR研究人员在其 对艾滋病毒治疗、预防、性别差异、对治疗的反应以及 免疫病理学景观

PROJECT SUMMARY/ABSTRACT –SYSTEMS BIOLOGY & BIOSTATISTICS CORE F The RUSTBELT CFAR Systems Biology & Biostatistics Core F provides support to a wide range of CFAR investigators by offering new multi-omic technologies, advanced biostatistics and computational analysis of “big data,” a stronger foundation in functional immunology studies, and a comprehensive ‘omic data management and training platform. The key mission of Core F is to provide systems biology approaches that allow for integration of different layers of host immune responses, from molecular triggers of immune responses to transcriptional signatures and interaction with resident microbiota. We will continue to support functional immunology studies that remain fundamental to HIV research. The Core assists investigators with research rigor and reproducibility through implementation of best practices for study design, analysis, and public sharing of results. In Aim 1 Core F will support genomics and microbiome research in HIV immunology and pathogenesis by providing users with a robust technological platform for transcriptional analysis by RNA-seq as well as deep sequencing in clinical and animal models of HIV infection. In Aim 2 Core F provides training and consultation in functional immunology as well as centralized access to state-of-the-art immunologic technologies, protocols, and training for the isolation, manipulation, culturing, and expansion of primary immune cells from blood and tissue, thereby supporting the RUSTBELT CFAR’s HIV transmission, gender research, cure, and pathogenesis translation objectives. In Aim 3 Core F facilitates all aspects of systems biology studies, by offering a centralized data acquisition, management, and reporting structure for all cohorts and ‘omics, using high-capacity assay and analysis workflows as well as publicly available ‘omic data to build publications and databases. Lastly, in Aim 4 This service function includes the collection and preparation of ‘omic and functional data for coordination and distribution to investigators, collecting associated de-identified patient annotations needed for analysis, and study publication. Core F Core F performs biostatistical correlate analysis to identify key drivers of protective immune responses, assist in designing validation strategies and writing manuscripts, and provide the metadata and data standardization pipelines for upload of results to public databases. By providing fundamental immune modeling support paired with multi-omic methodologies to identify key drivers or biomarkers of the host-virus interface in HIV, Core F will foster the complete systems biology approach necessary to support CFAR investigators in their translational research toward HIV cure, prevention, gender discrepancies, response to treatment, and the immunopathological landscape.